甲基红分光光度法测定头孢克肟  

Spectrophotometric Determination of Cefixime Using Methyl Red

作　　者：胡小明 高先明[2] 于洋 陈泰辉 HU Xiaoming;GAO Xianming;YU Yang;CHEN Taihui(School of Chemistry and Environmental Engineering,Pingdingshan University,Pingdingshan,Henan 467036,China;Henan Shenma Nylon Chemical Co Ltd,Pingdingshan,Henan 467000,China)

机构地区：[1]平顶山学院化学与环境工程学院,河南平顶山467036 [2]河南神马尼龙化工有限责任公司,河南平顶山467000

出　　处：《平顶山学院学报》2021年第2期39-42,共4页Journal of Pingdingshan University

摘　　要：研究了头孢克肟与甲基红的电荷转移反应,建立了快速测定头孢克肟的可见分光光度法.结果表明:电荷转移络合物的络合比为1∶1,最大吸收波长为525 nm.实验发现:荷移反应体系在35℃,反应时间为10 min时,效果最好.头孢克肟质量浓度在0.6~20 mg/L范围内服从比尔定律,线性相关系数为0.988 6.当头孢克肟浓度为8.00 mg/L时,6次测定结果的相对标准偏差为1.43%.该方法用于检测药物中头孢克肟的含量,加标回收率为97.25%~104%.The charge transfer reaction between cefixime and methyl red has been studied and a rapid method for determination of cefixime was developed. The results showed that the maximum absorption wavelength was525 nm when the composition ratio was 1 ∶1. It was found that the best effect was obtained when the charge transfer reaction system was at 35 ℃ for 10 minutes. Beer’s Law was obeyed in the range of 0. 6 ~ 20 mg/L of cefixime with correlation coefficient of 0. 988 6. The relative standard deviation was 1. 43%( n = 6) when the concentration of cefixime was 8 mg/L. The proposed method was used in the determination of the content of cefixime in samples with satisfactory results. The recoveries of cefixime were 97. 25% and 104%.

关 键 词：甲基红 头孢克肟 分光光度法 荷移反应 

分 类 号：R927.2[医药卫生—药学] R978.11