ELA对骨髓间充质干细胞氧化应激损伤的保护作用  

Protective Effect of ELA on Oxidative Stress Injuryof Bone Marrow Mesenchymal Stem Cells

作　　者：符佳颖 陈旭翔 许岱诗 刘欣 杨欢 周长青 吴海东 曾朝涛[1] 吴浩[1] 郑光辉[1] 王彤 FU Jia-ying;CHEN Xu-xiang;XU Dai-shi;LIU Xin;YANG Huan;ZHOU Chang-qing;WU Hai-dong;ZENG Chao-tao;WU Hao;WANG Tong(Sun Yat-sen Memorial Hospital of Sun Yal-sen University,510120;The Eighth Affiliated Hospital of Sun Yat-sen University)

机构地区：[1]中山大学孙逸仙纪念医院,510120 [2]中山大学附属第八医院

出　　处：《岭南急诊医学杂志》2021年第1期1-3,共3页Lingnan Journal of Emergency Medicine

基　　金：国家自然科学基金(81070125,81270213,81670306);广东省自然科学基金(2017A030313503);广东省科技计划项目(No:2010B031600032,2014A020211002);广州市科技计划项目(201806020084);高校基本科研业务费中山大学资助计划(13ykzd16,17ykjc18);深圳市科创委基金(JCYJ20190808101405466)。

摘　　要：目的:观察ELABELA(ELA)对过氧化氢(H_(2)O_(2))诱导骨髓间充质干细胞(Mesenchymal stem cells),ELA(500 nM,1μM,5μM,10μM)组。利用H_(2)O_(2)诱导MSCs凋亡模型,即H_(2)O_(2)组细胞加入含400μM H_(2)O_(2)的无血清培养基,在37℃条件下培养6小时。MTS法检测细胞增殖及细胞活力,DCFH-DA荧光探针法检测细胞内活性氧(ROS)含量。结果:与Control组相比,H_(2)O_(2)组细胞的存活能力显著降低(P<0.05),伴有细胞内活性氧水平明显升高(P<0.05);与H_(2)O_(2)组相比,ELA组细胞的存活率显著增加(P<0.05),且细胞内活性氧水平显著降低(P<0.05),其中以5μMELA治疗对MSCs的保护作用最佳。结论:ELA能够减轻过H_(2)O_(2)诱导的MSCs氧化应激损伤,其中以5μM浓度ELA的保护效应以达到最佳。Objective:Toobserve the effect of ELABELA(ELA)on the injury of bone marrow mesenchymal stem cells(MSCs)induced by hydrogen peroxide(H_(2)O_(2))and investigate the optimum concentration.Methods:The MSCs cultured in vitro were divided into Control group,H_(2)O_(2) group,ELA(500 nM,1μM,5μM,10μM)groups.The apoptosismodel ofMSCswas induced by H_(2)O_(2),that is,the H_(2)O_(2) group cells were exposed to serum-free medium containing400μM H_(2)O_(2) and cultured at 37℃for 6 hours.MTS was used to detect cell proliferationandviability.DCFH-DA fluorescence probe method was detected to evaluate the level of intracellular reactive oxygen species(ROS).Results:Compared with the Control group,the cell viability of the H_(2)O_(2) group was remarkably reduced(P<0.05),accompanied by a significant increase in intracellularROSlevel(P<0.05).Compared with the H_(2)O_(2) group,the cell survival rate of ELA groupssignificantly increased(P<0.05),and the level of intracellular ROS was dramatically reduced(P<0.05).Among them,treatment with 5μM ELA had the best protective effect on MSCs.Conclusion:ELA can reduce the oxidative stress damage of MSCs induced by H_(2)O_(2),and the protective effect of ELA at a concentration of 5μM is optimal.

关 键 词：ELABELA 骨髓间充质干细胞 过氧化氢 氧化应激 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]