微流控拉曼检测芯片的制备与应用  

Preparation and Application of Microfluidic Raman Detection Chip

作　　者：李春赫 马卓晨 胡昕宇 朱琳 韩冰[2] 张永来 Li Chunhe;Ma Zhuochen;Hu Xinyu;Zhu Lin;Han Bing;Zhang Yonglai(State Key Laboratory of Integrated Optoelectronics,College of Electromic Science and Engineering,Jilin University,Changchu,Jilin 130012,China;State Key Laboratory of Precision Testing Technology and Inustruments,Department of Precision Instruments,Tsinghua University,Beijing 100084,China)

机构地区：[1]吉林大学电子科学与工程学院,集成光电子学国家重点实验室,吉林长春130012 [2]清华大学精密仪器系,精密测试技术及仪器国家重点实验室,北京100084

出　　处：《中国激光》2021年第2期127-150,共24页Chinese Journal of Lasers

基　　金：国家重点研发计划(2017YFB1104600);国家自然科学基金(61935008,61775078,61905087,61590930);吉林省科技发展规划(20180101061JC);中国博士后科学基金面上资助项目(2018M640122)。

摘　　要：微流控芯片系统具有高效率、低损耗、高安全系数、高灵敏度等优势,表面增强拉曼散射(SERS)光谱具有灵敏度高以及指纹效应强等优点。从两方面对微流控拉曼检测芯片进行综述:微流控芯片通道和SERS基底的制备以及微流控拉曼检测芯片的集成与应用。最后讨论了SERS微流控芯片在便携化应用方面的挑战和机遇,并对整个领域的未来发展方向与前景进行了展望。Significance Microfluidic chips incorporate basic operation units such as sample preparation,reaction,separation,and detection on a microchip,revealing great potential in the chemical and biological analysis.Compared with the traditional macroscopic large-volume systems,microfluidic chips feature the advantages of high efficiency,low loss,high safety factor,and high sensitivity.As a high-throughput micro-scale analysis device,the microfluidic chip system has shown significant potential in the highly sensitive detection of various chemical and biological molecules.So far,several detection methods such as ultraviolet-visible absorption,plasma atomic emission spectrometry,inductive coupling,mass spectrometry,chemiluminescence,laser-induced fluorescence,thermal lens microscopy,and biosensors,have been successfully applied to microfluidic systems.Among them,the surface enhanced Raman scattering(SERS)method as a unique detection technology was also introduced to the detection of microfluidic chips and rapidly developed in the past decade.Because SERS is a fingerprint feature map with rich spectral lines,it has high sensitivity,fast speed,and non-contact.Combined with the characteristics of the microfluidic chip,the SERS detection method shows several unique advantages:the laser spot is small and can be directly focused on the tiny channel of the microfluidic chip;the high sensitivity is especially suitable for the requirement of a small amount of reagents in the microfluidic chip;it has no direct contact with the reaction reagents,and it has no interference to the reaction system;with fingerprint spectral characteristics,it can be used to analyze and identify the mixture in the reaction system.Progress In this review,we will discuss and study the development of SERS microfluidic chips from two parts.The first part is the preparation of microfluidic chip channels and integrated SERS substrates.The second one is the application of SERS integrated microfluidic chips.Figure 1 shows the overall discussion ideas of this arti

关 键 词：光学制造 表面增强拉曼散射 微流控芯片 微通道 拉曼增强基底 激光加工 检测分析 

分 类 号：O437[机械工程—光学工程]