神效散外用对急性软组织损伤后肿瘤坏死因子-α、白细胞介素-1β的影响  

Shenxiao San Powder for External Use on the Influence of TNF-αand IL-1βafter Acute Soft Tissue Injury

作　　者：王爱国 谷福顺[2] 郑昆仑[2] 陆军 赵允[1] 王晓辉[3] WANG Ai-guo;GU Fu-shun;ZHENG Kun-lun(Affiliated Hospital of Tianjin Academy of TCM,Tianjin 300120,China)

机构地区：[1]天津市中医药研究院附属医院,天津300120 [2]天津中医药大学第二附属医院,天津300250 [3]天津市医药科学研究所,天津300020

出　　处：《中国中西医结合外科杂志》2021年第2期198-201,共4页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

基　　金：天津市中医药领域重点项目(2017007);天津市中医药研究院附属医院院内科研基金(2020004)。

摘　　要：目的:探究神效散外用对急性软组织损伤后肿瘤坏死因子-α(TNF-α)和白细胞介素-β(IL-1β)的影响。方法:将50只大鼠随机分为5组,神效散高、中、低剂量组,空白敷贴阴性对照组,扶他林阳性对照组,建立局部软组织损伤模型,连续给药7 d后,检测血清和肌肉组织中TNF-α和IL-1β的含量。结果:第4天时,每组血清中TNF-α、IL-1β含量与阴性对照组均有统计学差异(P<0.05);第4天时,高剂量组与中剂量组肌肉组织中TNF-α、IL-1β含量与阴性对照组有统计学差异(P<0.05);第7天时中剂量组和低剂量组血清TNF-α、IL-1β含量与阴性对照组有统计学差异(P<0.05);第7天时,中剂量组肌肉组织中TNF-α、IL-1β含量与阴性对照组有统计学差异(P<0.05)。结论:中剂量组神效散外用对急性软组织局部损伤模型有较好的抗炎作用,其机制可能是使损伤组织中TNF-α、IL-1β含量降低。Objective To investigate Shenxiao San Powder for external use on the influence of TNF-αand IL-1βafter acute soft tissue injury.Methods The rats were randomly divided into 5 groups,namely the experimental group with high,medium and low doses of Shenxiao San Powder,the control group with negative blank application and the positive control group with Votalin.The local soft tissue injury model was established and the levels of TNF-αand IL-1βin serum and muscle tissues were observed after 7 consecutive days of administration.Results On the 4th day,there were statistically significant differences in serum levels of TNF-αand IL-1βin each group compared with the negative control group(P<0.05).On the 4th day,the levels of TNF-αand IL-1βin the muscle tissues of the high-dose group and the middle-dose group were significantly different from those of the negative control group(P<0.05).On the 7th day,the serum levels of TNF-αand IL-1βin the medium dose group and the low dose group were significantly different from the negative control group(P<0.05).On the 7th day,there were statistically significant differences between the levels of TNF-αand IL-1βin the muscle tissue of the medium dose group and the negative control group(P<0.05).Conclusion The medium dose Shenxiao San Powder has a good anti-inflammatory effect on the model of acute local soft tissue injury.The mechanism may be related to the decrease of TNF-αand IL-1βin the injured tissue.

关 键 词：急性软组织损伤 神效散外用 局部损伤模型 肿瘤坏死因子-Α 白细胞介素-1Β 

分 类 号：Q95-33[生物学—动物学] R258.6[医药卫生—中医内科学]