柔性表面增强拉曼光谱芯片制备  

Fabrication of Flexible Surface-Enhanced Raman Spectroscopy Chip

作　　者：杨峰 文枰[1,2,3] 张志强 李丹阳 陈李[1,2] 李顺波 徐溢[1] Yang Feng;Wen Ping;Zhang Zhiqiang;Li Danyang;Chen Li;Li Shunbo;Xu Yi(Key Laboratory of Optoelectronic Technology and Systems,Ministry of Education,College of Optoelectronic Engineering,Chongqing University,Chongqing 400044,China;State Key Laboratory of Transducer Technology,Chinese Academy of Sciences,Shanghai 200050,China;School of Intelligent Manufacturing,Sichuan University of Arts and Science,Dazhou,Sichuan 635000,China)

机构地区：[1]重庆大学光电工程学院光电技术与系统教育部重点实验室,重庆400044 [2]中国科学院传感器技术国家重点实验室,上海200050 [3]四川文理学院智能制造学院,四川达州635000

出　　处：《中国激光》2021年第1期190-199,共10页Chinese Journal of Lasers

基　　金：国家重点研发计划(2018YFB2002302);国家自然科学基金(61971074);重庆大学中央高校基本科研业务费专项(2019CDYGYB003);四川文理学院校级课题(2019PT002Z)。

摘　　要：柔性表面增强拉曼光谱(SERS)基底具有灵活形变的特点,适合不规则曲面的原位检测,甚至可以直接进行擦拭取样的检测。对不同反射率的衬底进行仿真分析和实验测试,可以看到衬底的反射率对拉曼信号的收集有极大的影响。在波长为532 nm的光激发铝箔具有高反射率,因此选择铝箔作为衬底,采用银溶胶滴铸法制备柔性SERS芯片。实验通过控制溶剂成分,利用表面张力梯度引起向内马兰哥尼(Marangoni)流动以抑制咖啡环的产生,可以改善纳米粒子的分布均匀性。拉曼测试结果表明,SERS芯片的增强因子高达1.32×10^(8),对R6G溶液的检测限低至1×10^(-11)mol,同时芯片表现出良好的信号均匀性。Objective Surface-enhanced Raman spectroscopy(SERS)can provide fingerprint information on target molecules with high detection sensitivity without being affected by water,which makes it an attractive non-destructive analysis technology.The SERS active substrate is key part of inspection applications.Therefore,a significant research effort has been devoted to the development of a stable,uniform,and repeatable SERS substrate.Unlike rigid SERS substrate,flexible SERS substrate can be flexibly deformed,which is convenient for in situ detection on irregular curved surfaces and can even be used for wipe sampling detection directly.However,the characteristics of the flexible substrate itself significantly impact the SERS performance,and the commonly used sol drop casting method to prepare SERS active substrates is significantly affected by the"coffee ring"effect.Because the effect caused uneven distribution of nanoparticles,uniformity of the SERS signal is affected.In this paper,simulation analysis and experimental research on the reflectivity of the substrate are carried out,and optimization of the substrate is performed to suppress the"coffee ring"effect.A high-throughput and array-type flexible SERS chip with excellent performance is successfully fabricated,which has application potential in biomedicine,food safety,environmental pollution,and other detection fields.Methods To study the influence of substrate reflectivity on SERS performance,the Raman signal intensities of different substrates are compared and analyzed by simulation(COMSOL Multiphysics)and experimental tests.To eliminate the"coffee ring"effect generated from the evaporation of nanoparticle suspensions,we control the solvent composition by adding a certain proportion of ethylene glycol in Ag sol,and use inward Marangoni flow induced by the surface tension gradient,which ensure uniform deposition of nanoparticles.The array detection unit of the SERS chip is fabricated on aluminum foil through laser printing.Then,Ag sol mixed liquid is dropped into the d

关 键 词：表面光学 表面增强拉曼光谱 柔性芯片 银纳米颗粒 咖啡环效应 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]