松花粉对脂多糖诱导小鼠学习记忆功能损伤的保护作用  

Protective effect of pine pollen on lipopolysaccharide-induced learning and memory impairment in mice

作　　者：蒋陆霞[1] 王娟[1] 付小彬[1] Jiang Luxia;Wang Juan;Fu Xiaobin(Department of Pharmacy,Liaocheng People's Hospital,Liaocheng 252000,Shandong Province,China)

机构地区：[1]聊城市人民医院药学部,山东省252000

出　　处：《中国基层医药》2021年第3期430-434,共5页Chinese Journal of Primary Medicine and Pharmacy

基　　金：山东省自然科学基金项目(ZR2015HL031)。

摘　　要：目的研究松花粉对脂多糖(LPS)诱发小鼠学习记忆功能损伤的保护作用和机制。方法将60只小鼠采用数字表法随机分为四组:正常对照组(n=15)、模型组(n=15)、松花粉低剂量组(500 mg/kg,n=15)和松花粉高剂量组(1000 mg/kg,n=15)。小鼠侧脑室一次性注射LPS建立小鼠学习记忆功能损伤模型,通过水迷宫实验检测小鼠的学习记忆能力,同时测定小鼠海马超氧化物歧化酶(SOD)的活性,谷胱甘肽(GSH)和丙二醛(MDA)的含量,同时检测小鼠海马白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)含量,以及多巴胺(DA)和去甲肾上腺素(NE)的含量。结果与正常对照组小鼠相比,模型组小鼠避暗实验潜伏期[(134.80±33.89)s比(282.20±17.43)s]明显缩短(t=4.23,P<0.01),错误次数[(4.00±1.58)次比(1.20±1.30)次]显著升高(t=2.85,P<0.01)。给予松花粉治疗后可显著提升小鼠避暗实验潜伏期[(189.40±27.21)s、(213.40±21.26)s比(134.80±33.89)s](t=3.21、4.38,均P<0.05),降低小鼠的错误次数[(1.60±1.44)次、(1.40±1.44)次比(4.00±1.58)次](t=5.12、6.42,均P<0.05)。同时,模型组小鼠海马中SOD的活性,GSH、DA、NE的含量较正常对照组小鼠[(7.59±1.77)kU/g比(39.90±6.37)kU/g,(3.49±0.13)mmol/g比(6.37±0.14)mmol/g,(418.42±2.57)ng/L比(586.37±3.64)ng/L,(187.20±5.41)ng/L比(298.42±2.32)ng/L]显著降低(t=3.67、8.23、2.23、3.65,均P<0.05),MDA、IL-6和TNF-α的含量[(8.79±0.82)mmol/g比(2.62±0.16)mmol/g,(48.07±5.56)ng/L比(18.76±1.42)ng/L,(87.20±4.31)ng/L比(22.42±3.39)ng/L]显著升高(t=7.45、2.67、4.35,P<0.05或P<0.01)。给予松花粉治疗后,与模型组小鼠相比,SOD的活性,GSH、DA和NE的含量[(18.80±2.39)kU/g、(28.70±2.36)kU/g比(7.59±1.77)kU/g,(5.04±0.36)mmol/g、(5.45±0.17)mmol/g比(3.49±0.13)mmol/g,(488.37±3.46)ng/L、(506.29±5.72)ng/L比(418.42±2.57)ng/L,(225.65±3.72)ng/L、(239.76±5.58)ng/L比(187.20±5.41)ng/L]显著升高(t=4.56、6.71,t=4.65、5.32,t=4.73、6.72,t=3.84、5.63,P<0.05或P<0.01),MDA、IL-6�Objective To investigate the protective effect of pine pollen on lipopolysaccharide(LPS)-induced learning and memory impairments in mice and the underlying mechanism.Methods Sixty mice were randomly divided into four groups(n=15/group):normal control,model,low-dose pine pollen(500 mg/kg)and high-dose pine pollen(1000 mg/kg).Mouse models of learning and memory impairment were established by lateral ventricle injection of LPS.The learning and memory abilities of mice were determined by the Morris water maze test.Superoxide dismutase(SOD)activity and glutathione(GSH)and malondialdehyde(MDA)levels in the hippocampus of mice were measured.Interleukin-6(IL-6),tumor necrosis factor-α(TNF-α),dopamine(DA),and norepinephrine(NE)levels in the hippocampus were also determined.Results The latency in the passive avoidance test in the model group was significantly shorter than that in the normal control group[(134.80±33.89)s vs.(282.20±17.43)s,t=4.23,P<0.01].The number of errors in the model group was significantly higher than that in the normal control group[(4.00±1.58)vs.(1.20±1.30)times,t=2.85,P<0.01].The latency in the passive avoidance test in the low-dose pine pollen(500 mg/kg)and high-dose pine pollen(1000 mg/kg)groups was significantly longer than that in the normal control group[(189.40±27.21)s or(213.40±21.26)s vs.(134.80±33.89)s,t=3.21,4.38,all P<0.05].The number of errors in the low-dose pine pollen(500 mg/kg)and high-dose pine pollen(1000 mg/kg)groups was significantly lower than that in the normal control group[(1.60±1.44)times or(1.40±1.44)times vs.(4.00±1.58)times,t=5.12,6.42,both P<0.05].SOD activity and GSH,DA and NE levels in the hippocampus in the model group were significantly decreased compared with the normal control group[SOD:(7.59±1.77)kU/g vs.(39.90±6.37)kU/g;GSH:(3.49±0.13)mmol/g vs.(6.37±0.14)mmol/g;DA:(418.42±2.57)ng/L vs.(586.37±3.64)ng/L;NE:(187.20±5.41)ng/L vs.(298.42±2.32)ng/L,t=3.67,8.23,2.23,3.65,all P<0.05].MDA,IL-6 and TNF-αlevels in the hippocampus in the normal control

关 键 词：松花粉 脂多糖类 学习 记忆 海马 氧化性应激 神经递质转运蛋白质类 炎症介导素类 小鼠 

分 类 号：R285.5[医药卫生—中药学]