石油烃降解菌降解性能及其对含油土壤的修复  

Degradation Performance of Petroleum Degrading Bacteria and Remediation of Oil Contaminated Soil

作　　者：李敏[1] 李婷婷[2] 彭湃[2] 王恩彪 孟垚 李彦成 侯欣园 杨思宇 魏小娜[1] LI Min;LI Ting-ting;PENG Pai;WANG En-biao;MENG Yao;LI Yan-cheng;HOUXin-yuan;YANG Si-yu;WEIXiao-na(Liaoning Shihua University,Fushun Liaoning 113001,China;Shenyang Research Institute of Chemical Industry,Shenyang Liaoning 110021,China)

机构地区：[1]辽宁石油化工大学,辽宁抚顺113001 [2]沈阳化工研究院有限公司,辽宁沈阳110021

出　　处：《辽宁化工》2021年第2期127-130,共4页Liaoning Chemical Industry

基　　金：国家自然科学基金青年基金项目(项目编号:41701563);辽宁石油化工大学引进人才科研启动基金(项目编号:2016XJJ-049);辽宁省大学生创新创业训练计划(项目编号:201910148039)。

摘　　要：考察了筛选出的3株石油降解菌C3 Pseudomonas putida、C4 Acinetobacter calcoaceticus、C5 Sphingomona sp.对石油烃的降解性能,研究了3株菌的复合菌、生物炭+复合菌、生物炭固定化复合菌等不同菌剂加入方式对含油土壤的修复效果。结果表明:3株菌均能以石油烃为唯一碳源和能源生长,C3、C4对饱和烃的去除能力较强,C5对芳香烃的去除能力较强,3株菌的复合菌对石油烃各组分的去除具有协同效果,30℃培养28d复合菌对石油烃的降解率达到79.73%;生物炭与复合菌联用对石油烃的去除具有协同效应,其去除效果CTB>TB+BC>TB;生物炭的加入有利于降解菌在土壤中的定殖,其中生物炭作为固定化载体加入土壤对降解菌的数量增加最为有利。Three kinds of petroleum degrading bacteria, Pseudomonas putida(C3), Acinetobacter calcoaceticus(C4) and sphingomona sp.(C5) were selected to degrade petroleum hydrocarbons. After testing their degradation abilities of the total petroleum hydrocarbons and its components, a microbial degradation flora was constructed. The degradation flora was then used in the remediation of petroleum-contaminated soil. The results demonstrated an obvious effect of three strains in the degradation of petroleum hydrocarbons. C3 and C4 were more susceptible to degrade saturated hydrocarbons while C5 was more inclined to remove aromatic hydrocarbon. After 28 days of incubation at 30 ℃, the degradation rate of hydrocarbons reached 79.73%,which demonstrated a synergistic effect on the removal of different hydrocarbons by the degradation flora. Besides, the combination of biochar and degradation flora showed a synergistic effect on the removal of petroleum hydrocarbon in the remediation tests. The removal efficiency was CTB > TB + BC > TB. It indicated that the addition of biochar was conducive to the survival of exogenous microorganisms in soil, and biochar as a immobilized carrier was the most favorable to increase the number of degradation bacteria.

关 键 词：石油烃降解菌 固定化 土壤修复 

分 类 号：TE991.3[石油与天然气工程—石油机械设备]