钙库操纵性钙通道蛋白在环磷酰胺诱导的膀胱过度活动症大鼠模型膀胱组织中的表达  

Expression of store-operated calcium channels in bladder tissue of rats with cyclophosphamide induced overactive bladder

作　　者：熊志 郝晓杰 高宏飞[2] XIONG Zhi;HAO Xiao-jie;GAO Hong-fei(Grade 2018,the First Clinical Medical College of Shanxi Medical University,Taiyuan,Shanxi 030001,China;Department of Urology,the First Hospital of Shanxi Medical University,Taiyuan,Shanxi 030001,China)

机构地区：[1]山西医科大学第一临床医学院,山西太原030001 [2]山西医科大学第一医院泌尿外科,山西太原030001

出　　处：《中华实用诊断与治疗杂志》2021年第3期226-229,共4页Journal of Chinese Practical Diagnosis and Therapy

基　　金：山西省留学回国人员科技活动择优资助项目[晋人社厅函(2017)397号]。

摘　　要：目的制备膀胱过度活动症(overactive bladder,OAB)大鼠模型,探讨钙库操纵性钙通道蛋白在OAB大鼠膀胱组织中的表达。方法30只健康雌性SD大鼠,随机分为模型组和对照组各15只。模型组单次腹腔注射环磷酰胺200mg/kg制备OAB模型,对照组腹腔注射等量生理盐水。2组腹腔注射后24h行尿流动力学检查,观察排尿间期、排尿阈值、基础膀胱压力和平均逼尿肌压力。尿流动力学检查后立即处死大鼠,取膀胱组织,采用Western blot法检测膀胱组织钙释放激活钙通道蛋白1(calcium release-activated calcium channel protein 1,Oria1)、基质交联分子1(stromal interacting molecule 1,STIM1)蛋白相对表达量,实时荧光定量PCR法检测Oria1mRNA、STIM1mRNA相对表达量。结果对照组储尿期及排尿期逼尿肌收缩平稳、未见明显不稳定收缩,模型组在储尿期出现明显不稳定收缩;模型组排尿间期[(217.8±176.7)s]短于对照组[(412.8±276.4)s](P<0.05),排尿阈值[(10.0±2.6)cm H_(2)O]低于对照组[(14.3±2.7)cm H_(2)O](P<0.05),基础膀胱压[(11.4±3.7)cm H_(2)O]高于对照组[(7.5±2.6)cm H_(2)O](P<0.05),平均逼尿肌压力[(54.8±20.7)cm H_(2)O]与对照组[(43.2±8.2)cm H_(2)O]比较差异无统计学意义(P>0.05)。模型组膀胱组织Orai1蛋白(0.36±0.21)及STIM1蛋白(0.63±0.46)相对表达量、Orai1mRNA(1.92±0.80)及STIM1mRNA(0.42±0.22)相对表达量均高于对照组(0.21±0.13、0.44±0.31、0.59±0.06、0.16±0.02)(P<0.05)。结论环磷酰胺诱导的OAB大鼠模型膀胱组织中钙库操纵性钙通道相关蛋白Orai1及STIM1表达升高。Objective To prepare rat models of overactive bladder(OAB)and to investigate the expression of store-operated calcium channels in the bladder tissue.Methods Thirty healthy female SD rats were randomly and equally divided into model group and control group.OAB models were established by intraperitoneal single injection of cyclophosphamide 200mg/kg in model group,and control group was intraperitoneally injected with the same amount of normal saline.Urodynamic detection was done 24hafter intraperitoneal injection to observe the voiding interval,voiding threshold,basal bladder pressure and mean detrusor pressure.The rats were sacrificed immediately after urodynamic detection to obtain the bladder tissue.Western blot was used to detect the expressions of calcium release activated calcium channel protein 1(Orai1)and stromal interacting molecule 1(STIM1)proteins in the bladder tissue.Real-time fluorescence quantitative PCR was used to detect the relative expressions of Oria1mRNA and STIM1mRNA.Results In control group,the detrusor contractions were stable during the period of urine storage and urination,and no obvious unstable contractions were found.In model group,obvious unstable contractions occurred during the period of urine storage.The voiding interval was shorter in model group((217.8±176.7)s)than that in control group((412.8±276.4)s)(P<0.05),the voiding threshold was lower in model group((10.0±2.6)cm H_(2)O)than that in control group((14.3±2.7)cm H_(2)O)(P<0.05),the basal bladder pressure was higher in model group((11.4±3.7)cm H_(2)O)than that in control group((7.5±2.6)cm H_(2)O)(P<0.05),and the mean detrusor pressure showed no significant difference between model group((54.8±20.7)cm H_(2)O)and control group((43.2±8.2)cm H_(2)O)(P>0.05).The relative expressions of Orai1and STIM1proteins and mRNAs were higher in model group(0.36±0.21,0.63±0.46,1.92±0.80,0.42±0.22)were higher than those in control group(0.21±0.13,0.44±0.31,0.59±0.06,0.16±0.02)(P<0.05).Conclusion The expressions of store-operated cal

关 键 词：膀胱过度活动症 大鼠 环磷酰胺 钙库操纵性钙通道蛋白 钙释放激活钙通道蛋白1 基质交联分子1 

分 类 号：R694[医药卫生—泌尿科学]