SD-QF-PCR对流产组织染色体数目畸变的检测  

Detection of chromosome aneuploidy in abortion fetus or chorionic villi using SD-QF-PCR

作　　者：孙雷 龙驹 樊祖茜 SUN Lei;LONG Ju;FAN Zuqian(Qinzhou Maternal and Child Health Hospital,Qinzhou,Guangxi 535000,China)

机构地区：[1]钦州市妇幼保健院,广西钦州535000

出　　处：《中国优生与遗传杂志》2021年第1期96-99,共4页Chinese Journal of Birth Health & Heredity

基　　金：国家自然科学基金(81660259)。

摘　　要：目的探索重复片段的定量荧光PCR技术(SD-QF-PCR)在检测自然流产胚胎或绒毛组织染色体数目畸变应用中的价值。方法采用已经验证过的SD-QF-PCR体系,对2016年11月至2020年5月共1920例流产胚胎或绒毛组织进行染色体数目的检测,并对结果进行统计分析。结果在1920例样本中,有105例存在母体组织污染导致无法检测,697例检出染色体数目异常,1118例未检出染色体数目异常。结论染色体数目畸变是导致自然流产的主要原因,因此,发生自然流产时,应及时行胚胎染色体的数目检测,以明确流产的病因。Objective To develop a novel method of testing aneuploidy in abortion fetus or chorionic villi using SD-QF-PCR method, and to report the clinical application of this method in practice. Methods The numbers of 1920 cases from November 2016 to May 2020 were detected by SD-QF-PCR method, The results were statistically analyzed. Results In 1920 samples, 105 cases were failed due to the maternal tissue pollution, 697 cases with abnormal chromosome number, and 1118 cases with common chromosome number. Conclusion chromosomal number aberration is the main cause of spontaneous abortion. Therefore, when spontaneous abortion occurs, it is necessary to detect the chromosome number aberration to determine the genetic cause of abortion.

关 键 词：SD-QF-PCR 自然流产 绒毛膜绒毛 染色体数目畸变 

分 类 号：R714.21[医药卫生—妇产科学]