Three-dimensional reconstruction and comparison of vacuolar membranes in response to viral infection  

作　　者：Xueting Wang Juncai Ma Xuejiao Jin Ning Yue Peng Gao Keith Ka Ki Mai Xian-Bing Wang Dawei Li Byung-Ho Kang Yongliang Zhang 

机构地区：[1]State Key Laboratory of Agro-Biotechnology and Ministry of Agriculture Key Laboratory of Soil Microbiology,College of Biological Sciences,China Agricultural University,Beijing 100193,China [2]State Key Laboratory of Agro-Biotechnology,School of Life Sciences,The Chinese University of Hong Kong,Hong Kong 999077,China

出　　处：《Journal of Integrative Plant Biology》2021年第2期353-364,共12页植物学报（英文版）

基　　金：supported by grants from the National Transgenic Science and Technology Program (2019ZX08010-003);the National Natural Science Foundation of China (31872637);Chinese Universities Scientific Fund (2020TC181);the Research Grants Council of Hong Kong (GRF14126116, GRF14121019, C4012-16E, C4002-17G, and Ao E/M-05/12);Cooperative Research Program for Agriculture Science & Technology Development (0109532019) Rural Development Administration, Republic of Korea。

摘　　要：The vacuole is a unique plant organelle that plays an important role in maintaining cellular homeostasis under various environmental stress conditions. However, the effects of biotic stress on vacuole structure has not been examined using three-dimensional(3D) visualization. Here, we performed 3D electron tomography to compare the ultrastructural changes in the vacuole during infection with different viruses. The 3D models revealed that vacuoles are remodeled in cells infected with cucumber mosaic virus(CMV) or tobacco necrosis virus A Chinese isolate(TNV-AC), resulting in the formation of spherules at the periphery of the vacuole. These spherules contain neck-like channels that connect their interior with the cytosol. Confocal microscopy of CMV replication proteins 1 a and 2 a and TNV-AC auxiliary replication protein p23 showed that all of these proteins localize to the tonoplast.Electron microscopy revealed that the expression of these replication proteins alone is sufficient to induce spherule formation on the tonoplast, suggesting that these proteins play prominent roles in inducing vacuolar membrane remodeling. This is the first report of the 3D structures of viral replication factories built on the tonoplasts. These findings contribute to our understanding of vacuole biogenesis under normal conditions and during assembly of plant(+) RNA virus replication complexes.

关 键 词：COMPARISON cucumber mosaic virus electron tomography SPHERULES tobacco necrosis virus A VACUOLE viral replication 

分 类 号：S432.41[农业科学—植物病理学]