脂肪来源间充质干细胞治疗神经损伤性勃起功能障碍的实验研究  

Experimental study on adipose-derived mesenchymal stem cell promoting the recovery of erectile dysfunction following cavernous nerve injury

作　　者：提运荣 杨孟波 张明[1,2] 肖冬冬 吕向国 卢慕峻 Ti yunrong;Yang mengbo;Zhang ming;Xiao dongdong;Lv xiangguo;Lu mujun(Department of Urology,Renji Hospital,Shanghai Jiaotong University School of Medicine,Shanghai,200127,China;Shanghai Institute of Andrology)

机构地区：[1]上海交通大学医学院附属仁济医院泌尿外科,上海200127 [2]上海市男科学研究所,上海200001

出　　处：《中国男科学杂志》2021年第1期32-37,共6页Chinese Journal of Andrology

摘　　要：目的建立大鼠神经损伤勃起功能障碍(CNI-ED)动物模型,通过在阴茎海绵体注射脂肪来源间充质干细胞(ASCs),探究ASCs对双侧海绵体神经损伤后勃起功能障碍的治疗效果和作用机制。方法实验动物分为3组,每组8只,分别为年龄匹配组(AMC组,假手术治疗),对照组(双侧海绵体损伤+海绵体注射PBS,PBS组),实验组(双侧海绵体损伤+海绵体注射ASCs,ASCs组)。手术后4周和12周,检测阴茎海绵体测压(ICP/MAP比值)和组织学结果。结果手术4周后,对照组大鼠的ICP/MAP比值明显低于AMC组(p<0.001),而实验组的ICP/MAP比值较对照组有显著提高(p<0.05);12周后,对照组和实验组大鼠ICP/MAP比值仍低于AMC组(p<0.001),但实验组显著高于对照组(p<0.05),提示ASCs治疗能够促进大鼠的勃起功能恢复。Masson染色结果表明,术后4周和12周对照组平滑肌/胶原纤维比值较AMC组明显降低(p<0.001),实验组平滑肌/胶原纤维比值高于对照组(p<0.05)。免疫荧光结果显示,术后4周和12周AMC组大鼠nNOS数量显著高于对照组和实验组(p<0.001),而实验组显著高于对照组(p<0.05)。12周后实验组的nNOS数量比4周有显著提高(p<0.05)。结论实验组在功能学(ICP/MAP比值)和形态学(平滑肌胶原纤维比值)及海绵体nNOS恢复上均优于对照组,证实海绵体注射ASCs能够促进CNI-ED大鼠勃起功能恢复,主要机制是促进海绵体神经再生和抗海绵体纤维化。Objective To establish rat model with erectile dysfunction caused by cavernous nerve injury(CNI-ED),and investigate the therapeutic effect of ASCs on cavernous nerve injury erectile dysfunction and its related mechanism.Methods All animals were divided into three groups:the age-matched control(AMC)group(sham operation group),the control group(bilateral cavernous injury+injection of PBS),the experiment group(bilateral cavernous injury+injection of ASCs).After 4 weeks,the cavernous pressure(ICP/MAP ratio)and histological results of cavernous body were detected.Results After 4 weeks of operation,the ICP/MAP ratio of the control group was significantly lower than that of the AMC group(P<0.001),while the ICP/MAP ratio of the experiment group was significantly higher than that of the control group(P<0.05);after 12 weeks,the ICP/map ratio of the control group and experiment group was still lower than that of the AMC group(P<0.001),but the value of the experiment group was significantly higher than that of the control group(P<0.05),suggesting that ASCs can promote the recovery of erectile function in rats.Masson staining demonstrated that the ratio of smooth muscle/collagen fiber in control group was significantly lower than that in AMC group at 4 and 12 weeks after operation(P<0.001),and the ratio of smooth muscle/collagen fiber in the experiment group was higher than that in control group(P<0.05).Immunofluorescence results showed that the number of nNOS in AMC group was significantly higher than that in control group and the experiment group at 4 and 12 weeks after operation(P<0.001),while that in the experiment group was significantly higher than that in control group(P<0.05).After 12 weeks,the number of nNOS in the experiment group was significantly higher than that in 4 weeks(P<0.05)Conclusion The experiment group were superior to the control group in function(ICP/MAP ratio),morphology(smooth muscle collagen fiber ratio)and recovery of nNOS in corpus cavernosum.It is confirmed that ASCs can promote the functional re

关 键 词：勃起功能障碍 脂肪来源间充质干细胞 海绵体神经损伤 

分 类 号：R698.1[医药卫生—泌尿科学]