绿原酸、咖啡酸苯乙酯及聚乙烯吡咯烷酮联合添加对猪冷冻精液品质的影响  

Effect of combined addition of chlorogenic acid, phenethyl caffeate and polyvinylpyrrolidone on quality of frozen semen in pigs

作　　者：王珺 钱芙蓉[2] 菅芯蕊 张帅 张志彬[2] 张绍萱 于永生[2] 李晓霞 李兆华[2] WANG Jun;QIAN Furong;JIAN Xinrui;ZHANG Shuai;ZHANG Zhibin;ZHANG Shaoxuan;YU Yongsheng;LI Xiaoxia;LI Zhaohua(Agricultural College,Yanbian University,Yanji 133002,China;Animal Husbandry and Veterinary Institute,Jilin Academy of Agricultural Science,Gongzhuling 136100,China;College of Animal Science and Technology,Jilin University,Changchun 130062,China;Collge of Animal Science and Technology,Jilin Agriculural Science and Technology University,Jilin 132109,China)

机构地区：[1]延边大学农学院,吉林延吉133002 [2]吉林省农业科学院畜牧兽医研究所,吉林公主岭136100 [3]吉林大学动物科学学院,长春130062 [4]吉林农业科技学院动物科技学院,吉林吉林132109

出　　处：《黑龙江畜牧兽医》2021年第4期43-48,153,154,共8页Heilongjiang Animal Science And veterinary Medicine

基　　金：长春市国际科技合作计划项目“猪精子抗冻性分子标记的研究”(12ZX73);吉林省农业科技创新工程项目“猪冻精品质相关基因的多态性研究”(C92072034)。

摘　　要：为了研究抗氧化剂绿原酸(CGA)、咖啡酸苯乙酯(CAPE)及冷冻保护剂聚乙烯吡咯烷酮(PVP)联合添加对猪冷冻精液品质的影响,试验采用手握法对3头新吉林黑猪进行精液采集,将品质最佳的精液平均分为7组,即对照组和试验Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ、Ⅵ组,其中对照组不添加抗氧化剂及冷冻保护剂,试验Ⅰ组添加100μmol/L CGA,试验Ⅱ组添加40μmol/L CAPE,试验Ⅲ组添加0.5 g/L PVP,试验Ⅳ组添加100μmol/L CGA和0.5 g/L PVP,试验Ⅴ组添加40μmol/L CAPE和0.5 g/L PVP,试验Ⅵ组添加100μmol/L CGA、40μmol/L CAPE和0.5 g/L PVP,进行液氮冷冻保存,30 d后解冻精液,测定精子活力、线粒体活率、DNA完整率、质膜完整率、顶体完整率、过氧化氢酶(CAT)活性、超氧化物歧化酶(SOD)活性、谷胱甘肽过氧化物酶(GSH-Px)活性、活性氧(ROS)水平及丙二醛(MDA)含量。结果表明:与对照组相比,添加100μmol/L CGA、40μmol/L CAPE和0.5 g/L PVP的冷冻精液的精子活力、质膜完整率、顶体完整率、线粒体活率、CAT活性、GSH-Px活性均极显著提高(P<0.01);DNA完整率、SOD活性均显著提高(P<0.05);ROS水平极显著降低(P<0.01);MDA含量显著降低(P<0.05)。说明在猪精液冷冻基础液中同时添加100μmol/L CGA、40μmol/L CAPE和0.5 g/L PVP可提高新吉林黑猪冷冻精液品质。To investigate the effects of the combination of antioxidant chlorogenic acid(CGA), phenethyl caffeate(CAPE) and cryoprotectant polyvinylpyrrolidone(PVP) on the quality of frozen semen in pigs, semen from 3 Xin Jilin Black pigs were collected by hand grip method in this experiment. The semen with the best quality was divided into 7 groups on average, namely control group(no antioxidant and cryoprotectant), experimental group I(add 100 μmol/L CGA), experimental group II(add 40 μmol/L CAPE);experimental group III(0.5 g/L PVP), experimental group IV(add 100 μmol/L CGA and 0.5 g/L PVP), experimental group V(add 40 μmol/L CAPE and 0.5 g/L PVP), experimental group VI(add 100 μmol/L CGA、40 μmol/L CAPE and 0.5 g/L PVP). After 30 d of frozen storage in liquid nitrogen, semen was thawed, and the sperm motility, mitochondrial activity rate, DNA integrity rate, plasma membrane integrity rate, acrosome integrity rate, catalase(CAT) activity, superoxide dismutase(SOD) activity, glutathione peroxidase(GSH-Px) activity, reactive oxygen species(ROS) level, and malondialdehyde(MDA) content of frozen semen were measured. The results showed that the sperm motility, plasma membrane integrity rate, acrosome integrity rate, mitochondrial activity rate, CAT activity and GSH-Px activity of frozen semen in adding 100 μmol/L CGA、40 μmol/L CAPE and 0.5 g/L PVP group were significantly improved compared with the control group(P<0.01);DNA integrity rate and SOD activity were significantly increased(P<0.05);the ROS level decreased significantly(P<0.01);and the MDA content decreased significantly(P<0.05). The results indicated that adding 100 μmol/L CGA、40 μmol/L CAPE and 0.5 g/L PVP to the frozen base solution of pig spermatozoa could improve the frozen semen quality of Xin Jilin Black pig.

关 键 词：新吉林黑猪 精液 绿原酸 咖啡酸苯乙酯 聚乙烯吡咯烷酮 精液品质 

分 类 号：S828.89[农业科学—畜牧学]