北山羊与绒山羊杂交F1代DQA1基因外显子2多态性与CDS区生物信息学分析  

Polymorphism of exon 2 and bioinformatics analysis of CDS of DQA1 in hybrids F1 generation of Ibex and Cashmere goat

作　　者：吕雪峰 许艳丽 胡昕 赛迪古丽·赛买提 王乐 LV Xuefeng;XU Yanli;HU Xin;Saidiguli·SAIMAITI;WANG Le(Insitute of Animal Husbandry Quality Standards,Xinjiang Academy of Animal Sciences,Umuqi 830046,China)

机构地区：[1]新疆畜牧科学院畜牧业质量标准研究所,乌鲁木齐830046

出　　处：《黑龙江畜牧兽医》2021年第4期49-54,154,155,共8页Heilongjiang Animal Science And veterinary Medicine

基　　金：新疆维吾尔自治区自然科学基金项目(2016D01A004)。

摘　　要：为了解北山羊杂交后代DQA1基因序列特征,以及DQA1基因多态性与血液免疫性状的关系,筛选出用于北山羊杂交后代抗病育种的遗传标记,试验通过PCR扩增和测序获得了北山羊与家养绒山羊杂交F1代DQA1基因CDS区,并对CDS区编码蛋白进行了生物信息学预测分析,同时采用限制性片段长度多态性(RFLP)分析DQA1基因外显子2的多态性,并与血液免疫性状进行了相关分析。结果表明:杂交F1代DQA1基因外显子2经AluⅠ酶切后共检出3种基因型,其中AC型白细胞和淋巴细胞数量显著高于BB型(P<0.05),AB型血红蛋白浓度显著高于BB型(P<0.05);DQA1基因CDS区长度为768 bp,编码255个氨基酸,表现为弱的亲水性,第23,24位氨基酸存在信号肽剪切位点,第218~240位有一个典型的跨膜螺旋区,二级结构主要由α-螺旋(16.08%)、β-折叠(25.49%)和无规卷曲(58.43%)组成。说明DQA1基因AC基因型与血液白细胞和淋巴细胞总数具有一定的相关性,其编码蛋白的生物信息学预测与其功能相一致,可作为抗病育种的遗传标记。In order to understand DQA1 sequence characteristics of Ibex hybrid offspring, and relationship between DQA1 polymorphism and blood immune trait, and to screen genetic markers for resistance breeding in hybrids F1 generation of Ibex, in this experiment, the CDS sequence of DQA1 gene in F1 generation of Ibex and domestic cashmere goat was obtained by PCR amplified and sequenced, the bioinformatics analysis of CDS was performed, and the polymorphism of exon 2 of DQA1 gene was analysed by restriction fragment length polymorphism(RFLP), and the relationship between the polymorphism of exon 2 and blood immune traits in DQA1 gene was analyzed. The results showed that three genotypes in exon 2 of DQA1 gene in F1 generation were detected by AluⅠ enzyme digestion, among which the number of leukocytes and lymphocytes of AC genotype were higher than that of BB genotype(P<0.05), and the hemoglobin content of AB genotype was higher than that of BB gene type(P<0.05). The CDS of DQA1 gene had a length of 768 bp, and encoded by 255 amino acids, which shows weak hydrophilicity. There were signal peptide cleavage sites at the 23~24 of amino acid sequence and a typical transmembrane helix region at 218~240 of amino acid sequence. The secondary structure of CDS was composed of α-helix(16.08%), β-fold(25.49%) and random coil(58.43%). The results indicated that the AC genotype of DQA1 gene was associated with the number of blood leukocytes and lymphocytes, and the bioinformatics prediction of the protein encoded by DQA1 gene was consistent with its function in DQA1 gene, which could be used as a genetic marker for resistance breeding.

关 键 词：北山羊 绒山羊 杂交F1代 DQA1 多态性 蛋白序列分析 生物信息学 

分 类 号：S827[农业科学—畜牧学]