Cytotoxicity of oleanane type triterpene from leaf extract of Pterospermum acerifolium (in vitro) and theoretical investigation of inhibitory signaling pathway  

作　　者：Mohd Rehan Shafiullah Sultanat 

机构地区：[1]Department of Chemistry,Aligarh Muslim University,Aligarh 202002,India

出　　处：《Chinese Herbal Medicines》2021年第1期124-130,共7页中草药（英文版）

摘　　要：Objective: To evaluate the cytotoxic activity of taraxerol isolated from the leaves of Pterospermum acerifolium and its EtOH extract against human breast, colon, and lung cancer cell lines and docking studies.Methods: The structures of the isolated compounds were elucidated by several spectroscopic methods such as 1H-NMR,13C-NMR, DEPT-135, COSY, HSQC, and HMBC. The extract and isolated compound were analyzed for cytotoxic activity on MDA-MB-231, BT-549, A-549, and SW-480 cancer cell lines by MTT assay. Molecular docking was performed on software such as Chem3D pro 12.0.2.1076, Discovery Studio Visualizer, Auto Dock Tools-1.5.6 and Auto dock vina.Results: The extract and isolated compound taraxerol both displayed excellent inhibitory activity(IC50:80 mg/mL for extract and 160 mg/mL for compound) on breast cancer cell(MDA-MB-231). The docking studies show a strong affinity with PI3K(-9.8 Kcal/mol) and m TOR(-10.0 Kcal/mol).Conclusion: The results confirm that the extract and compound exhibited strong cytotoxicity on the MDA-MB-231 cancer cell. So, the extract and the compound may be useful in human chemotherapies.

关 键 词：breast cancer MDA-MB-231 mTOR PI3K Pterospermum acerifolium(L.)Willd 

分 类 号：R285.5[医药卫生—中药学]