长链非编码RNA OIP5-AS1对miR-143介导的动脉粥样硬化血管内皮细胞的作用  

Effect of long-chain non-coding RNA OIP5-AS1 on microRNA-143-mediated atherosclerotic vascular endothelial cells

作　　者：侯丹丹[1] 王加璐[1] 赵久晗[1] HOU Dan-dan;WANG Jia-lu;ZHAO Jiu-han(Department of Neurosurgery,the First Affiliated Hospital of China Medical University,Shenyang 110001,China)

机构地区：[1]中国医科大学附属第一医院神经内科,辽宁沈阳110001

出　　处：《解剖科学进展》2021年第1期53-57,共5页Progress of Anatomical Sciences

基　　金：国家自然科学青年基金(81601129)。

摘　　要：目的探讨长链非编码RNA OIP5-AS1对miR-143介导的动脉粥样硬化血管内皮细胞的作用。方法首先收集2016年1月至2018年12月我院心内科收治的动脉粥样硬化患者50例,同期收集我院体检中心身体健康志愿者50例作为对照组,采用qPCR检测和比较外周血循环OIP5-AS1的表达水平差异。以人脐动脉内皮细胞(HUVEC)为研究对象,过表达OIP5-AS1,采用CCK-8检测HUVEC的增殖活性,采用流式细胞术检测HUVEC的凋亡率。根据starbase预测OIP5-AS1可与动脉粥样硬化的标志分子miR-143结合,进一步采用荧光素酶报告基因实验和RNA免疫沉淀实验进行验证。结果动脉粥样硬化患者外周血循环OIP5-AS1的含量明显低于健康人群(AUC=0.723, P<0.001)。OIP5-AS1过表达促进HUVEC细胞的增殖,HUVEC的血管结节数和分支长度均明显增高,HUVEC的凋亡率降低。过表达OIP5-AS1的HUVEC细胞,miR-143表达水平上调。miR-143 mimc与HUVECOIP5-mt共转染HUVEC细胞后,其荧光值明显低于miR-143 mimc与共转染HUVEC-OIP5-AS1-wt和对照组。结论过表达OIP5-AS1促进血管内皮细胞的生长和血管发生、抑制凋亡,通过竞争性拮抗动脉粥样硬化发生的高危因子miR-143发挥血管保护作用。Objective To investigate the effect of long-chain non-coding RNA OIP5-AS1 on miR-143-mediated atherosclerotic vascular endothelial cells. Methods Fifty patients with atherosclerosis were collected from January 2016 to December 2018. Fifty healthy volunteers from the physical examination center of our hospital were collected as control group. The expression of OIP5-AS1 in peripheral blood was detected by qPCR. were used as research objects. The proliferation ability of human umbilical artery endothelial cells(HUVEC) was detected by CCK-8, and the apoptotic rate of HUVEC was detected by flow cytometry. According to starbase’s prediction that OIP5-AS1 can bind to the marker of atherosclerosis, miR-143, the luciferase reporter assay and RNA immunoprecipitation assay were performed. Results The content of OIP5-AS1 in peripheral blood circulation was significantly lower in atherosclerotic patients than in healthy people(AUC = 0.723, P <0.001). The overexpression of OIP5-AS1 promoted the proliferation of HUVEC cells, increased the number of vascular nodules and branch length of HUVEC, and reduced the apoptotic rate of HUVEC. The expression level of miR-143 was increased in OIP5-AS1 overexpressed HUVEC cells. The fluorescence value of HUVEC-OIP5-AS1-mt and miR-143 mimc co-transfected HUVEC cells was significantly lower than that of co-transfected control group. Conclusion The overexpression of OIP5-AS1 promotes the growth of human umbilical vascular endothelial cells, inhibits angiogenesis and apoptosis, and play a protective role via competitive antagonism of the high-risk factor miR-143.

关 键 词：动脉粥样硬化 OIP5-AS1 MIR-143 血管内皮细胞 凋亡 

分 类 号：R543.1[医药卫生—心血管疾病]