香蕉种质资源超低温保存技术研究进展  

Advance on the Ultra-Low Temperature Preservation Technology of Banana Germplasm Resources

作　　者：李羽佳[1] 许竹叶 唐文[2] 许奕[1] 王安邦[1] 王笑一 李敬阳[1] 唐粉玲[1] LI Yujia;XU Zhuye;TANG Wen;XU Yi;WANG Anbang;WANG Xiaoyi;LI Jiangyang;TANG Fenling(Haikou Experimental Station,Chinese Academy of Tropical Agricultural Science/Engineering Technology Research Center of Health Banana Seedling Propagation of Hainan Province,Haikou 571101,Hainan;institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,Hainan)

机构地区：[1]中国热带农业科学院海口实验站/海南省香蕉健康种苗繁育工程技术研究中心,海南海口571101 [2]中国热带农业科学院热带生物技术研究所,海南海口571101

出　　处：《中国热带农业》2021年第2期49-53,共5页China Tropical Agriculture

基　　金：现代农业产业体系专项资金项目(CARS-31-02);海南省重点研发计划(ZDYF2019060);中国热带农业科学院基本科研业务费专项资金(1630092020002);中国热带农业科学院基本科研业务费专项资金(1630092020007)。

摘　　要：香蕉是一种大型草本植物,多数品种没有种子或单性结实,通常以无性繁殖方式为主。由于其生殖特征,难以通过传统杂交育种的方法来进行品种改良,而多是采用体细胞突变的方法来选育新品种。因此,香蕉资源的多样性对香蕉新品种改良尤为重要。但由于常(低)温离体保存、田间活体保存方法易受到生物或非生物胁迫等影响因素限制费力、耗能,其种质资源的长期有效保存对育种工作而言极具挑战性,而超低温保存技术是一种长期有效的保存方法。本研究主要针对香蕉超低温保存的不同方法,总结了影响香蕉超低温保存效果的几个关键因素。The banana is a large herb.Most of bananas are free of seeds or parthenocarpy,usually in the form of asexual reproduction.Due to its reproductive characteristics,it is difficult to improve the variety through traditional hybrid breeding methods,and somatic mutation methods are mostly used to select new varieties.Therefore,the diversity of banana resources is particularly important for the improvement of new banana varieties.However,the long-term effective preservation of germplasm resources is very challenging for breeding work,because normal(low)temperature in vitro preservation and field living preservation are vulnerable to biotic and abiotic stress and other factors,which are laborious and energy-consuming.Ultra-low temperature preservation technology is a long-term effective preservation method.This paper mainly focused on the different methods of banana cryopreservation,and summarized several key factors affecting the ultra-low temperature preservation effect of bananas.

关 键 词：香蕉 种质资源 超低温保存 PVS 

分 类 号：S668.1[农业科学—果树学]