槲皮素对痛风模型大鼠血清NETs和淋巴细胞水平的影响  

Effect of quercetin on serum NETs and lymphocyte levels in gout model rats

作　　者：铁宁[1] 李刚 白丽杰[1] 王静[1] 赵静[1] 王勇[1] TIE Ning;LI Gang;BAI Lijie;WANG Jing;ZHAO Jing;WANG Yong(Department of Rheumatology and Immunology,The Affiliated Hospital of Inner Mongolia Medical University,Hohhot,Inner Mongolia 010059,China;School of Pharmacy,Inner Mongolia Medical University,Hohhot,Inner Mongolia 010059,China)

机构地区：[1]内蒙古医科大学附属医院风湿免疫科,内蒙古呼和浩特010059 [2]内蒙古医科大学药学院,内蒙古呼和浩特010059

出　　处：《国际检验医学杂志》2021年第8期996-998,1002,共4页International Journal of Laboratory Medicine

基　　金：内蒙古自治区自然科学基金项目[2017MS(LH)0839,2018MS08097]。

摘　　要：目的分析槲皮素对痛风模型大鼠血清中性粒细胞胞外诱捕网(NETs)和淋巴细胞水平的影响。方法将30只大鼠采用随机数字表法分为对照组、模型组、槲皮素组,每组10只。对模型组和槲皮素组大鼠进行造模,造模成功后槲皮素组大鼠给予200 mg/kg槲皮素灌胃,对照组及模型组同期灌胃等体积的蒸馏水。给药前后依照炎症指数评定准则和Goderre功能障碍指数对大鼠急性炎症状况进行评估;使用足容积测量仪检测足趾肿胀度;给药第1天和第7天分析血清中淋巴细胞和游离DNA/中性粒细胞胞外诱捕网(cf-DNA/NETs)水平。结果槲皮素干预1周后,槲皮素组大鼠功能障碍指数、炎症指数和足趾肿胀度均明显低于模型组,且差异有统计学意义(P<0.05);给药后3组大鼠血清中淋巴细胞、cf-DNA/NETs水平差异有统计学意义(P<0.05),其中模型组最高,对照组最低,且两两比较差异均有统计学意义(P<0.05)。结论槲皮素干预痛风模型大鼠后可有效降低血清NETs和淋巴细胞水平。Objective To analyze the effect of quercetin on serum neutrophil extracellular traps(NETs)and lymphocyte levels in gout model rats.Methods Thirty rats were divided into control group,model group and quercetin group by random number table method,10 rats in each group.Rats in model group and quercetin group were modeled.After successful modeling,the rats in the quercetin group were given 200 mg/kg quercetin by gavage,and the rats in the control group and the model group were given the same volume of distilled water by gavage at the same time.Acute inflammation of rats was evaluated before and after intervention according to the inflammatory index evaluation criteria and Goderre dysfunction index.The degree of toe swelling was measured with a foot volume meter.Serum lymphocyte and cf-DNA/NETs levels were analyzed on the 1st and 7th day of administration.Results After one week of quercetin intervention,the disability index,inflammation index and toe swelling of the quercetin group were significantly lower than the model group,and the difference was statistically significant(P<0.05).After administration,there were significant differences in lymphocytes and cf-DNA/NETs between the three groups of rats(P<0.05),with the model group being the highest and the control group being the lowest,and the differences between the three groups were statistically significant(P<0.05).Conclusion Quercetin could effectively reduce the level of serum NETs and lymphocytes after intervention in gout model rats.

关 键 词：槲皮素 痛风 中性粒细胞胞外诱捕网 淋巴细胞 

分 类 号：R593.2[医药卫生—内科学]