土壤干旱及复水对侧柏叶绿素荧光参数的影响  

Effects of Drought and Rewatering on Chlorophyll Fluorescence Parameters of Platycladus orientalis

作　　者：张玉玉 王进鑫[2,3] 马戌 吕国利 冯树林 ZHANG Yuyu;WANG Jinxin;MA Xu;Lü Guoli;FENG Shulin(Institute of Soil and Water Conservation,Northwest A&F University,Yangling,Shaanxi 712100,China;College of Natural Resources and Environment,Northwest A&F University,Yangling,Shaanxi 712100,China;Key Laboratory of Plant Nutrition and the Agri-environment in Northwest China,Ministry of Agriculture,Yangling,Shaanxi 712100,China)

机构地区：[1]西北农林科技大学水土保持研究所,陕西杨凌712100 [2]西北农林科技大学资源环境学院,陕西杨凌712100 [3]农业部西北植物营养与农业环境重点实验室,陕西杨凌712100

出　　处：《水土保持研究》2021年第2期242-247,255,共7页Research of Soil and Water Conservation

基　　金：国家自然科学基金(31670713);国家重点研发计划项目课题(2017YFC0504402);榆林市林业科技计划项目(K403021528)。

摘　　要：为了研究不同程度土壤干旱及旱后复水对侧柏幼苗叶绿素荧光参数的影响,以盆栽1年生侧柏幼苗为试验材料,设置4个水分梯度[土壤相对含水量(RSWC)为:40%,52.16%,70%,87.84%],以充分供水(RSWC为100%)为对照,经干旱胁迫60 d后复水,测定了复水前及复水后2,24,48,72 h叶绿素荧光参数。结果显示:随着土壤干旱胁迫程度的加剧,侧柏幼苗PSⅡ的最大光化学量子产量Fv/Fm、相对光合电子传递速率ETR和实际光化学量子产量Yield总体上呈现下降的趋势,而非光化学淬灭系数NPQ则呈现上升的趋势,其中土壤相对含水量为40%的重度胁迫Fv/Fm,ETR和Yield分别比对照下降1.1%,4.5%,4.9%,而NPQ比对照增加22.6%。复水后叶绿素荧光都得到了恢复,在复水24 h除70%的处理其他处理Fv/Fm达到最大,其中重度胁迫40%的处理比对照上升3.1%。在复水48 h各胁迫处理Yield,ETR达到了最大值,40%的重度胁迫分别比对照上升1.0%,1.2%。由此可知,干旱胁迫尤其是重度胁迫,虽然对侧柏幼苗PSⅡ有一定的破坏,对光合作用有一定程度的影响,但复水后各指标都得到了相应的恢复。To study the effects of different degrees of soil drought stress and rehydration on chlorophyll fluorescence parameters of Platycladus orientalis seedlings,one-year-old Platycladus orientalis seedlings were used as experimental materials,and four water gradients[relative soil water content(RSWC):40%,52.16%,70%,87.84%]were set,100%RSWC(with adequate water supply)was kept as control.Drought stress was imposed for 60 days and then the plants were rewatered up to 100%RSWC.The chlorophyll fluorescence parameters were measured before rewatering and after 2,24,48 and 72 hours of rewatering.The maximum photochemical quantum yield(Fv/Fm),relative photosynthetic electron transfer rate(ETR)and actual photochemical quantum yield(Yield)of Platycladus orientalis PSⅡshowed the downward trend with the increasing water stress,while the non-photochemical quenching coefficient(NPQ)showed an upward trend.In case of 40%RSWC,Fv/Fm,ETR and Yield decreased by 1.1%,4.5%and 4.9%,respectively,whereas NPQ increased by 22.6%compared with the control.After rewatering,chlorophyll fluorescence parameters of Platycladus orientalis were restored in all the treatments to varying extent,Fv/Fm reached the maximum in the treatments(except 70%RSWC)after 24 hours of rewatering,and in 40%RSWC,Fv/Fm increased 3.1%compared with that of the control.In contrast,Yield and ETR reached the maximum in all the treatments after 48 hours of rewatering,and in 40%RSWC,Yield and ETR increased by 1.0%and 1.2%,respectively,compared to the control.Drought stress,especially severe stress(40%RSWC)had a certain damaging effect to PSⅡof Platycladus orientalis and a certain degree of influence on photosynthesis,but after rewatering,the indicators could be restored correspondingly.

关 键 词：侧柏 叶绿素荧光参数 干旱胁迫 旱后复水 

分 类 号：Q945.78[生物学—植物学]