硬粒小麦-长穗偃麦草附加系、代换系和易位系的创制  

Creation of disomic addition,substitution and translocation lines of durum wheat-Thinopyrum elongatum

作　　者：段亚梅 罗贤磊 陈士强 高勇[2] 陈建民 戴毅[1] DUAN Ya-Mei;LUO Xian-Lei;CHEN Shi-Qiang;GAO Yong;CHEN Jian-Min;DAI Yi(Institute of Agricultural Science and Technology Development/Joint International Research Laboratory of Agriculture&Agri-Product Safety of MOE,Yangzhou University,Yangzhou 225009,Jiangsu,China;College of Bioscience and Biotechnology/Co-Innovation Center for Modern Production Technology of Grain Crops,Yangzhou University,Yangzhou 225009,Jiangsu,China;Institute of Agricultural Sciences,Lixia River Region,Yangzhou 225009,Jiangsu,China)

机构地区：[1]扬州大学农业科技发展研究院/教育部农业与农产品安全国际联合实验室,江苏扬州225009 [2]扬州大学生物科学与技术学院/江苏高校现代谷物生产协作中心,江苏扬州225009 [3]江苏省里下河地区农业科学研究所,江苏扬州225009

出　　处：《作物学报》2021年第7期1402-1414,共13页Acta Agronomica Sinica

基　　金：江苏省自然科学基金项目(BK20180908,BK20181213);扬州市自然科学基金项目(YZ2018097)资助。

摘　　要：通过小麦与长穗偃麦草远缘杂交创制附加系、代换系及易位系是小麦遗传改良中利用长穗偃麦草优良基因的重要途径。本研究将长穗偃麦草特异分子标记、染色体计数、基因组原位杂交(GISH)及非变性原位杂交(ND-FISH)等多种方法相结合,对硬粒小麦Langdon(AABB)与小偃麦8801(AABBEE)的杂交后代群体进行分子细胞学鉴定,创制出硬粒小麦-长穗偃麦草3E、6E染色体双体附加系Du-DA3E和Du-DA6E,硬粒小麦-长穗偃麦草1E(1B)染色体双体代换系Du-DS1E(1B)以及硬粒小麦-长穗偃麦草1AS-1EL染色体易位系Du-T1AS·1EL。创制的4个种质中长穗偃麦草染色体均能稳定遗传,这不仅增加了硬粒小麦-长穗偃麦草附加系和代换系的类型,还为后续利用长穂偃麦草优良基因改良小麦提供了特殊种质资源。The establishment of chromosome addition,substitution and translocation lines through distant hybridization between wheat and Thinopyrum elongatum is an important approach to utilize useful genes of Th.elongatum in wheat improvement.Based on chromosome-specific molecular markers,chromosome counting,genome in situ hybridization(GISH),and non-denaturing fluorescence in situ hybridization(ND-FISH),several stable addition,substitution or translocation lines were identified from a cross between Triticum turgidum ssp.durum Langdon(AABB)and Trititrigia 8801(AABBEE).Two 3E and 6E disomic addition lines Du-DA3E and Du-DA6E of durum wheat-Th.elongatum,a 1E(1B)disomic substitution line Du-DS1E(1B),and a 1AS-1EL disomic translocation line Du-T1AS·1EL were created in this study.The chromosome-segments of Th.elongatum could be inherited stably in these new lines,which not only increased the types of addition and substitution lines of durum wheat-Th.elongatum,but also provided special germplasm resources for the follow-up use of excellent genes of Th.elongatum in wheat improvement.

关 键 词：硬粒小麦 长穗偃麦草 附加系 代换系 易位系 

分 类 号：S512.1[农业科学—作物学]