云南、浙江、内蒙古禾本科植物内生真菌多样性研究  

Diversity of endophytic fungi associated with plants of Poaceae from Yunnan,Zhejiang and Inner Mongolia

作　　者：刘蔚廷 陈家杰 冯佳威 夏晨阳 邵寅霄 朱逸骁 刘峰 蔡焕满 杨锴斌 章初龙[1] LIU Wei-Ting;CHEN Jia-Jie;FENG Jia-Wei;XIA Chen-Yang;SHAO Yin-Xiao;ZHU Yi-Xiao;LIU Feng;CAI Huan-Man;YANG Kai-Bin;ZHANG Chu-Long(Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province,Institute of Biotechnology,College of Agriculture and Biotechnology,Zhejiang University,Hangzhou,Zhejiang 310058,China;Administrative Bureau of Naban River Watershed National Nature Reserve,Jinghong,Yunnan 666100,China;Administrative Bureau of Fengyang Mountain-Baishanzu National Nature Reserve,Qingyuan,Zhejiang 323020,China)

机构地区：[1]浙江大学农业与生物技术学院生物技术研究所浙江省作物病虫生物学重点实验室,浙江杭州310058 [2]纳板河国家级自然保护区管理局,云南景洪666100 [3]凤阳山-百山祖国家级自然保护区管理局,浙江庆元323020

出　　处：《菌物学报》2021年第3期502-513,共12页Mycosystema

基　　金：国家自然科学基金面上项目(31870010)。

摘　　要：从云南、浙江和内蒙古的30属禾本科植物分离到1821株内生真菌,根据ITS rDNA系统发育分析和ITS预测真菌的阈值,将这些菌株鉴定为3门10纲34目216属,其中子囊菌门5纲26目192属,担子菌门3纲6目21属,毛霉门2纲2目3属。粪壳菌纲和座囊菌纲为主要优势纲,相对频率分别为54.8%和30.9%;座囊菌纲的格孢腔菌目和粪壳菌纲的肉座菌目、巨座壳目、小丛壳目、炭角菌目是主要优势目,相对频率分别为26.7%、12.6%、12.1%、11.3%和9.9%。主要优势纲和主要优势目在不同纬度地区的分布有明显差异。在鉴定的216个属级分类单元中,7个已知属Colletotrichum、Alternaria、Fusarium、Diaporthe、Penicillium、Arthrinium、Trichoderma和1个分类地位未定的属Pleosporales incertae sedis type12的相对频率大于2%,除了这个分类地位未定的属以外,7个已知属都是广泛分布的真菌;云南、浙江和内蒙古的内生真菌组成和多样性差异明显,二地或三地共有的属仅58个,且云南禾本科植物的内生真菌多样性程度最高,内蒙古禾本科植物的内生真菌多样性程度最低;根部和地上部组织的内生真菌组成和多样性差异明显,两者共有的属仅53个,地上部组织的内生真菌多样性高于根部组织。因此,进一步深入调查不同区域,特别是热带和亚热带的禾本科植物内生真菌将丰富我们对禾本科植物内生真菌多样性的认识。One thousand eight hundred and twenty-one isolates of endophytic fungi were isolated from 30 genera of Poaceae collected in Yunnan,Zhejiang and Inner Mongolia of China.They were identified by ITS rDNA sequence analysis and threshold for fungal delimitation.The sum total of 216 genera in 34 orders of 10 classes of 3 phyla were obtained,including 192 genera in 26 orders of 5 classes of Ascomycota,21 genera in 6 orders of 3 classes of Basidiomycota and 3 genera in 2 orders of 2 classes of Mucoromycota.Sordariomycetes and Dothideomycetes were the dominant classes with the relative frequency of 54.8%and 30.9%,respectively.Pleosporales of Dothideomycetes and Hypocreales,Magnaporthales,Glomerellales and Xylariales of Sordariomycetes were the dominant orders with the relative frequency of 26.7%,12.6%,12.1%,11.3%and 9.9%,respectively.The distribution of the dominant classes and orders were significantly different in regions with different latitude.Among the 216 genera identified,the relative frequency of seven known genera,Colletotrichum,Alternaria,Fusarium,Diaporthe,Penicillium,Arthrinium,Trichoderma,and a taxonomically uncertain genus,Pleosporales incertae sedis type 12,were over 2%.All the seven known genera were widely distributed.The community composition and diversity of endophytic fungi in Yunnan,Zhejiang and Inner Mongolia varied significantly,and only 58 genera were common when comparison was made between the two regions or among the three regions.The diversity of endophytic fungi in Yunnan was the highest,while that in Inner Mongolia was the lowest.The composition and diversity of endophytic fungi in roots and shoots varied significantly.Only 53 genera were common in roots and shoots,and the diversity of endophytic fungi in shoots was higher than that in roots.Further investigation in other regions,especially in tropical and subtropical regions,is necessary for enriching our understanding of the diversity of endophytic fungi in plants of Poaceae in China.

关 键 词：禾本科 内生真菌 多样性 

分 类 号：Q949.32[生物学—植物学]