冻干人尿中镉标准物质的研制  

Development of the certified reference material of cadmium in human urine(freeze-dried)

作　　者：潘亚娟[1] 张敬[1] 张福钢[1] 闫慧芳[1] Pan Yajuan;Zhang Jing;Zhang Fugang;Yan Huifang(National Institute of Occupational Health and Poison Control,China CDC,Beijing 100050,China)

机构地区：[1]中国疾病预防控制中心职业卫生与中毒控制所,北京100050

出　　处：《中华劳动卫生职业病杂志》2021年第3期220-223,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：科技基础性工作专项-公共卫生领域重要标准物质与应用规范研究(2014YF211000)。

摘　　要：目的研制冻干人尿中镉标准物质。方法采集正常人尿经处理后作为基质,通过加标、混匀、分装、冷冻干燥制备而成;经均匀性检验、稳定性考察后,由7家实验室采用2种方法进行合作定值,并进行不确定度评定。结果研制的标准物质最小取样量为0.20 ml,2个水平的均匀性检验值F分别为1.69和1.49,差异无统计学意义(P>0.05)。冻干标准物质在≤4℃下保存可稳定12个月,复溶后的标准物质溶液在4℃条件下可稳定7 d。2个水平的标准量值及不确定度分别为(5.34±0.34)μg/L和(15.47±0.72)μg/L(k=2)。结论研制的标准物质各项指标符合国家相关要求,可用于人尿中镉检测方法验证及相关样品检测。Objective To develop the certified reference material of cadmium in human urine(freeze-dried).Methods Urine were collected from normal human,and then the urine samples were prepared by adding standard,mixing,packing and freeze-drying.After homogeneity test and stability test,the urine samples were determined by two methods in seven independent laboratories,the uncertainty of the certified reference material was evaluated.Results Take at least 0.20 ml solution of the certified reference material per use,and the homogeneity is good.The freeze-dried materials can be kept stable for 12 months at≤4°C,and the re-dissolved reference materials can be stabilized for 7 days at 4°C.The standard values and uncertainty of the two levels were(5.34±0.34)μg/L and(15.47±0.72)μg/L,respectively.Conclusion The standards met the requirements of national standards,and can be used to verify the methods of detecting cadmium in human urine and to detect the samples.

关 键 词：镉 尿 人 标准物质 不确定度 

分 类 号：R115[医药卫生—公共卫生与预防医学] TQ421.31[化学工程]