玉米芯菌酶协同发酵饲料的制备工艺条件  

Response Surface Methodology to Optimize the Processing Parameters for Producing Corncob Fermentation Feed by Bacteria Coupled Fermentation with Enzymes

作　　者：林标声[1] 严建彬 钟志龙 罗建 周孝琼[1] LIN Biaosheng;YAN Jianbin;ZHONG Zhilong;LUO Jian;ZHOU Xiaoqiong(Longyan University;Yizhitai Biotechnology(Longyan)Co.Ltd;Longyan Zhenggao Biotechnology Co.Ltd;Fujian Longyan Jinhe Animal Feed Co.Ltd.,Longyan,Fujian 364000,China)

机构地区：[1]龙岩学院生命科学学院 [2]易之泰生物科技(龙岩)有限公司 [3]龙岩正高生物科技有限公司 [4]福建龙岩金和动物饲料有限公司,福建龙岩364000

出　　处：《龙岩学院学报》2021年第2期55-60,共6页Journal of Longyan University

基　　金：福建省科技厅农业科技引导性项目(2020N0033);龙岩经开区(高新区)奇迈科技创新基金项目(2018JKQ04);福建省教育厅中青年项目(科技)(JAT190733);福建省龙岩市科技计划农业类重点项目(2019LYF9020)。

摘　　要：采用响应面分析法中的Plackett-Burman、Box-Behnken设计优化玉米芯菌酶协同发酵饲料的制备工艺条件,并对发酵前后的产品性能进行测定分析。结果表明:玉米芯菌酶协同发酵的最优工艺参数为玉米芯691.75 g/kg、复合益生菌菌液接种量质量分数2.01%、NSP酶(非淀粉多糖酶)0.71 g/kg。菌酶协同发酵后,玉米芯中大分子物质利用效率增加,蛋白质转化效率明显提高,产生了明显的酒香味和乳酸味,产品色泽、手感、质地也发生了明显改善,为制备发酵预混饲料提供了参考依据。To study the optimum process conditions of corncob fermentation by bacteria coupled with enzymes,the Plackett-Burman,Box-Behnken design of response surface analysis method was used and the product properties before and after fermentation were measured and analyzed.The results revealed that the optimal conditions of corncob fermentation by bacteria coupled with enzymes was corncob 691.75 g/kg,compound probiotic liquid inoculation amount 2.01%,NSP enzyme(Non-starch polysaccharide enzyme)0.71 g/kg.After fermentation by bacteria coupled with enzymes,the utilization efficiency of macromolecules matter in corncob increased,and the protein conversion efficiency also increased obviously.Corncobs fermentation feed exhibited a deeper color following fermentation,which produced a transparent wine-like and lactic acid scent,and the color and texture of the product changed obviously.The research in this paper can provide a reference for the preparation of fermented premixed feed.

关 键 词：响应面分析法 玉米芯 菌酶协同发酵 工艺条件 

分 类 号：S816.6[农业科学—饲料科学]