miR-320a-5p靶向GINS2调控甲状腺鳞癌细胞SW579凋亡的机制研究  

Study on the mechanism of miR-320A-5P targeting GINS2 to regulate apoptosis of thyroid squamous cell carcinoma cell line SW579

作　　者：袁正泰 倪志强[1] 刘欢[1] 王永恒[1] 黄湘俊[1] YUAN Zheng-tai;NI Zhi-qiang;LIU Huan;WANG Yong-heng;HUANG Xiang-jun(First Affiliated Hospital of Hunan University of Traditional Chinese Medicine,Changsha 410007,China)

机构地区：[1]湖南中医药大学第一附属医院胃肠甲状腺血管外科,湖南长沙410007

出　　处：《生物技术》2021年第1期32-37,共6页Biotechnology

摘　　要：[目的]探讨miR-320a-5p调控甲状腺鳞癌细胞SW579凋亡的潜在机制。[方法]使用miR-320a-5p mimics和miR-320a-5p inhibitor甲状腺鳞癌细胞SW579中过表达或敲低miR-320a-5p,检测细胞的凋亡水平。通过miRDB,Human Target Scan,RNAhybrid软件分析和荧光素酶报告系统分析miR-320a-5p的底物。通过siRNA和质粒分别敲低和过表达底物,检测甲状腺鳞癌细胞SW579的凋亡水平。[结果]过表达miR-320a-5p后,甲状腺鳞癌细胞SW579的凋亡水平上升(P <0.05);敲低miR-320a-5p后,甲状腺鳞癌细胞SW579的凋亡水平下降(P <0.05)。miRDB,Human Target Scan,RNAhybrid软件分析发现miR-320a-5p潜在靶向多个基因。过表达miR-320a-5p后,发现甲状腺鳞癌细胞SW579中GINS2的表达水平下降(P <0.05);敲低miR-320a-5p后,发现甲状腺鳞癌细胞SW579中GINS2的表达水平上升(P <0.05)。miR-320a-5p靶向GINS2的3’端非编码区。敲低GINS2后,发现甲状腺鳞癌细胞SW579的凋亡上升(P <0.05)。过表达GINS2后,甲状腺鳞癌细胞SW579的凋亡下降(P <0.05)。[结论]miR-320a-5p通过靶向GINS2 mRNA的3’端非编码区,抑制了GINS2的翻译,最终抑制了甲状腺鳞癌细胞SW579的凋亡。[Objective] To explore the potential mechanism of miR-320 a-5 p in regulating the apoptosis of thyroid squamous cell carcinoma SW579.[Method] Use miR-320 a-5 p mimics and miR-320 a-5 p inhibitor to overexpress or knock down miR-320 a-5 p in SW579 thyroid squamous cell carcinoma cells to detect cell apoptosis. Analyze the substrate of miR-320 a-5 p by miRDB,Human Target Scan,RNAhybrid software analysis and luciferase reporter system. Knockdown and overexpression of substrates by siRNA and plasmid respectively to detect the level of apoptosis in thyroid squamous cell carcinoma SW579.[Result]After overexpression of miR-320 a-5 p,the apoptotic level of squamous thyroid cancer cell SW579 increased( P <0. 05);after knocking down miR-320 a-5 p,the apoptosis level of squamous thyroid cancer cell SW579 decreased( P < 0.05). miRDB,Human Target Scan,RNAhybrid software analysis found that miR-320 a-5 p potentially targets multiple genes. After over-expression of miR-320 a-5 p,the expression level of GINS2 in thyroid squamous cell carcinoma SW579 was found to decrease( P < 0. 05);after knocking down miR-320 a-5 p,it was found that the expression level of GINS2 in thyroid squamous cell SW579 increased( P < 0. 05). miR-320 a-5 p targets the 3’-terminal non-coding region of GINS2. After knocking down GINS2,it was found that the apoptosis of thyroid squamous cell carcinoma SW579 increased( P < 0. 05). After overexpression of GINS2,the apoptosis of thyroid squamous cell carcinoma SW579 decreased( P < 0. 05). [Conclusion] miR-320 a-5 p inhibits the translation of GINS2 by targeting the 3’ non-coding region of GINS2 mRNA,and finally inhibits the apoptosis of thyroid squamous cell carcinoma SW579.

关 键 词：miR-320a-5p GINS2 甲状腺鳞癌 SW579 凋亡 

分 类 号：Q784[生物学—分子生物学]