高通量测序研究不同程度间歇性低氧致大鼠肠道微生物菌群改变  

High throughput sequencing for studying changes of intestinal microflora in rats caused by different degrees of intermittent hypoxia

作　　者：李雯[1,2] 赵丹[1,2] 万自芳 张湘燕[1,2] LI Wen;ZHAO Dan;WAN Zifang;ZHANG Xiangyan(Guizhou Medical University,Guiyang,Guizhou 550004,China;Department of Respiratory and Critical Care Medicine,Guizhou Provincial People′s Hospital/Key Laboratory of Pulmonary Immune Diseases,Guizhou Provincial People′s Hospital,Guiyang,Guizhou 550002,China)

机构地区：[1]贵州医科大学,贵阳550004 [2]贵州省人民医院呼吸与危重症医学科/贵州省人民医院肺脏免疫性疾病重点实验室,贵阳550002

出　　处：《重庆医学》2021年第7期1087-1093,共7页Chongqing medicine

基　　金：贵州省科技计划项目(黔科[2016]支撑2907);贵州省人民医院青年基金项目(GZSYQN[2016]16号)。

摘　　要：目的研究不同程度间歇性低氧(CIH)导致大鼠肠道菌群改变。方法分别建立4周、8周CIH大鼠模型与对照组,留取各组大鼠粪便样品,利用Illumina MiSeq PE300平台进行高通量测序,测定所有粪便细菌的16SrRNA基因V3、V4区序列,使用Qimme软件分析菌群物种分类、物种多样性指数、样本间差异和各组生物学标志物。结果高通量测序及相关分析显示,低氧状态下大鼠肠道菌群较对照组大鼠发生明显改变。间歇性低氧增加了α多样性(shannon指数、simpson指数,P<0.05),引起肠道菌群的改变(β多样性的非参数多元方差分析,P<0.05)。间歇性低氧暴露的大鼠较对照组表现出更多的梭菌目、毛螺菌科、瘤胃球菌科等和更少的乳酸杆菌。结论间歇性缺氧改变了大鼠粪便微生物群的组成和多样性,使得肠道菌群紊乱,进而引发一系列疾病的发生。Objective To study the changes of rat intestinal microflora caused by different degrees of intermittent hypoxia.Methods The 4-week and 8-week intermittent hypoxic rat models and the control group rats were established respectively.The fecal samples of each group were collected.The high-throughput sequencing was performed by using the Illumine MiSeq PE300 platform.The sequences of V3 and V4 regions of 16SrRNA gene of all fecal bacteria were determined.The species classification,species diversity index,differences among samples and biomarkers of each group were analyzed with the Qimme software.Results The high-throughput sequencing and correlation analysis showed that the rat intestinal flora in hypoxia status had significant change compared with the control group.The intermittent hypoxia increasedαdiversity(shannon index,simpson index,P<0.05),and caused the changes of intestinal flora(nonparametric multivariate analysis ofβdiversity,P<0.05).The rats exposed to intermittent hypoxia showed more clostridiales,lachnospiraceae,ruminococcus,and fewer lactobacillus compared with those in the control group.Conclusion Intermittent hypoxia changes the composition and diversity of fecal microbiota,which leads to the disorder of intestinal microbiota and the occurrence of a series of diseases.

关 键 词：间歇性低氧 肠道菌群紊乱 高通量测序 大鼠 

分 类 号：R563[医药卫生—呼吸系统]