基于点击化学的壳聚糖水凝胶的制备及性能  

Preparation and Properties of Chitosan Hydrogels Based on Click Chemistry

作　　者：徐思诗 郎美东[1] XU Sishi;LANG Meidong(School of Materials Science and Engineering,East China University of Science and Technology,Shanghai 200237,China)

机构地区：[1]华东理工大学材料科学与工程学院,上海200237

出　　处：《材料科学与工程学报》2021年第2期224-229,共6页Journal of Materials Science and Engineering

基　　金：国家重点研发计划资助项目(2016YFC1100703)。

摘　　要：本研究首先在甲磺酸中用丙烯酰氯对壳聚糖(CS)进行接枝改性,通过一步法合成了水溶性丙烯酰基壳聚糖(CS-AC)。之后,在光引发剂I2959和紫外光(UV)照下,以二硫苏糖醇(DTT)为交联剂制备了基于巯基-烯点击化学的CS-AC/DTT快速交联水凝胶。红外光谱(FTIR)和核磁共振氢谱(^(1)H-NMR)结果定性和定量地证明了100%左右的双键接枝率。与CS-CC-CS自交联水凝胶40min以上的交联时间相比,CS-AC/DTT点击化学水凝胶最短能在10min之内就凝胶完全,此时双键/巯基投料比为1∶0.5。测试表明该水凝胶具有5-10KPa左右的强度以及高达1415%的溶胀率。此外,水凝胶对小鼠成纤维细胞(NIH-3T3)无明显毒性,在物理负载了莫匹罗星后对耐甲氧西林金葡萄球菌(MRSA)具有良好的抗菌性。A water soluble acryloyl chitosan(CS-AC)was synthesized through modifying chitosan(CS)with acryloyl chloride in methylsulfonic acid.Under UV exposure,a fast crosslinking CS-AC/DTT hydrogel was then prepared based on thiol-ene click chemistry using dithithitol(DTT)as crosslinker and I2959 as photoinitiator.Infrared spectroscopy(FTIR)and nuclear magnetic resonance(^(1)H-NMR)results qualitatively and quantitatively proved the vinyl grafting rate of around 100%.Comparing with the gelation time over 40 min of self-crosslinking hydrogel CS-CC-CS,that of click chemistry hydrogel CS-AC/DTT was shortened within 10 min with a vinyl/thiol feed ratio of 1∶0.5.Characterization results demonstrated the gel strength of 5-10 KPa and excellent swelling rate up to 1415%.Furthermore,cytotoxicity and antibacterial tests of hydrogels exhibited excellent cytocompatibility towards NIH-3 T3 cells and antibacterial properties towards resistant staplylococcus aureus methicillin-resistant staplylococcus aureus(MRSA).The above results reveal the potential application in drug loading and antibiosis for the synthesized hydrogel materials.

关 键 词：壳聚糖水凝胶 丙烯酰氯 巯基-烯点击化学 抗菌 

分 类 号：O636.1[理学—高分子化学]