荧光银纳米团簇的生物合成及其在痕量六价铬检测中的应用  

Synthesis of fluorescent silver nanoclusters for trace Cr(Ⅵ)detection

作　　者：田野[1] 娄虎 姬彦飞 董欣欣 赵熙明 张杰[1] Ye Tian;Hu Lou;Yanfei Ji;Xinxin Dong;Ximing Zhao;Jie Zhang(Key Laboratory of Saline-alkali Vegetation Ecology Restoration,Ministry of Education,College of Life Sciences,Northeast Forestry University,Harbin 150040,Heilongjiang Province,China;School of Forestry,Northeast Forestry University,Harbin 150040,Heilongjiang Province,China)

机构地区：[1]东北林业大学生命科学学院,东北盐碱植被恢复与重建教育部重点实验室,黑龙江哈尔滨150040 [2]东北林业大学林学院,黑龙江哈尔滨150040

出　　处：《微生物学报》2021年第3期740-749,共10页Acta Microbiologica Sinica

基　　金：国家自然科学基金(J1210053);哈尔滨市科技创新人才项目(2017RALXJ010)。

摘　　要：【目的】利用季也蒙毕赤酵母ZJC-1合成银纳米团簇并用于痕量Cr(Ⅵ)的检测。【方法】使用经耐银驯化的季也蒙毕赤酵母ZJC-1生物合成荧光银纳米团簇,并对其结构和荧光性能进行了表征,探究Cr(Ⅵ)对银纳米团簇荧光的选择性猝灭作用,建立了银纳米团簇荧光强度与Cr(Ⅵ)浓度的线性关系。同时还考察了体系p H和其他金属离子对Cr(Ⅵ)检测的影响。【结果】Cr(Ⅵ)浓度在一定的范围内(1–80μmol/L)与银纳米团簇荧光强度(F_0–F)/F_0有着良好的线性关系(R~2=0.9821),线性方程为(F_0–F)/F_0=0.0054×C_(cr(Ⅵ))+0.1876,检测限为184 nmol/L(信噪比为3)。利用该方法检测实际水样(松花江、马家沟河)中的Cr(Ⅵ),回收率介于97.73%–102.88%之间。【结论】以季也蒙毕赤酵母ZJC-1为还原剂和稳定剂,制备了具有较好荧光性能的水溶性银纳米团簇,基于Cr(Ⅵ)对银纳米团簇荧光的选择性猝灭作用,建立了一种快速且灵敏检测痕量Cr(Ⅵ)的新方法,并成功地应用于松花江、马家沟河水样中Cr(Ⅵ)的测定,在分析检测领域中具有良好的应用前景。[Objective]Silver nanoclusters were prepared using Pichia guilliermondii ZJC-1 for the detection of trace Cr(Ⅵ).[Methods]Fluorescent silver nanoclusters were synthesized using Pichia guilliermondii ZJC-1 that was acclimated to silver.The structures and fluorescence properties of silver nanoclusters were characterized.Then we explored the selective quenching effect of Cr(Ⅵ)on the silver nanoclusters fluorescence.We also established a linear relationship between silver nanoclusters fluorescence intensity and the concentration of Cr(Ⅵ).The effect of pH and other metal ions on the detection of Cr(Ⅵ)was also examined.[Results]There was a good linear relationship between Cr(Ⅵ)and the fluorescence intensity of silver nanoclusters(R2=0.9821).The detection limit was 184μmol/L and the signal to noise ratio(SNR)was 3.Using this method to detect Cr(Ⅵ)in actual water samples(Songhua River,Majiagou River)the recovery rate was between 97.73%and 102.88%.[Conclusion]Silver nanoclusters with good fluorescence properties can detect trace concentrations of Cr(Ⅵ)simply and rapidly.

关 键 词：银纳米团簇 生物合成 荧光猝灭 Cr(Ⅵ) 

分 类 号：O657.3[理学—分析化学] X832[理学—化学]