MicroRNA-155在原发性免疫性血小板减少症患儿外周血单个核细胞中的表达及意义  

Expression and significance of MicroRNA-155 in peripheral blood mononuclear cells of children with primary immunological thrombocytopenia

作　　者：散琴 崔莹莹[1] 柯盈月 SAN Qin;CUI Ying-ying;KE Ying-yue(Department of Clinical Laboratory,Shiyan Maternal and Child Health Care Hospital,Shiyan 442000,Hubei,CHINA)

机构地区：[1]十堰市妇幼保健院检验科,湖北十堰442000

出　　处：《海南医学》2021年第7期867-870,共4页Hainan Medical Journal

摘　　要：目的探讨MicroRNA-155在原发性免疫性血小板减少症(ITP)患儿外周血单个核细胞(PBMC)中的表达及其临床意义。方法选取十堰市妇幼保健院2015年2月至2019年6月收治的83例原发性ITP患儿纳入病例组,另选取同期于我院接受体检的健康儿童80例纳入健康组。比较两组受检者PBMC中的MicroRNA-155相对表达量及血小板计数(PLT)、细胞因子[干扰素-γ(IFN-γ)、白细胞介素-2(IL-2)、白细胞介素-4(IL-4)、白细胞介素-10(IL-10)]和T细胞亚群水平,采用Pearson相关性分析MicroRNA-155相对表达量与PLT、细胞因子及T细胞亚群水平的相关性。结果病例组患儿PBMC中MicroRNA-155相对表达量、Th1及血清IFN-γ、IL-2水平分别为4.31±1.04、(26.34±2.11)%、(151.23±5.02)pg/mL、(72.05±1.39)pg/mL,明显高于健康组的1.56±0.31、(11.52±2.20)%、(94.57±3.29)pg/mL、(42.37±1.28)pg/mL;而PLT、Treg、Th2及血清IL-4、IL-10水平分别为(45.62±18.55)×10^(9)/L、(3.60±0.75)%、(1.26±0.51)%、(24.92±1.05)pg/mL、(141.28±1.67)pg/mL,明显低于健康组的(198.73±25.21)×10^(9)/L、(3.98±0.37)%、(1.72±0.24)%、(42.37±1.72)pg/mL、(196.03±0.25)pg/mL,差异均有统计学意义(P<0.05);经Pearson相关性分析结果显示:PBMC中MicroRNA-155相对表达量与PLT(r=-0.683)、IL-4(r=-0.611)、IL-10(r=-0.520)、Treg(r=-0.522)、Th2(r=-0.572)水平均呈负相关(P<0.05),而与IFN-γ(r=0.634)、IL-2(r=0.545)、Th1(r=0.821)水平均呈正相关(P<0.05)。结论MicroRNA-155在ITP患儿PBMC中存在明显高表达,且与PLT、IL-4、IL-10、Treg及IFN-γ、IL-2、Th1、Th2水平均存在一定相关性,具有作为ITP患儿治疗靶点的潜力。Objective To investigate the expression and clinical significance of microRNA-155 in peripheral blood mononuclear cells(PBMC)of children with primary immune thrombocytopenia(ITP).Methods Eighty-three patients with primary ITP admitted to Shiyan Maternal and Child Health Hospital from February 2015 to June 2019 were included in the study group,and 80 healthy children who received physical examination in our hospital during the same period were included in the health group.The relative expression of microRNA-155,platelet count(PLT),cytokines(interferon-γ[IFN-γ],interleukin-2[IL-2],interleukin-4[IL-4],interleukin-10[IL-10]),and T cell subsets in PBMC of the two groups were compared.Pearson correlation was used to analyze the correlation between the relative expression of microRNA-155 and PLT,cytokines,and T cell subsets.Results The relative expression of microRNA-155,Th1,serum IFN-γ,and IL-2 in PBMC were 4.31±1.04,(26.34±2.11)%,(151.23±5.02)pg/mL,and(72.05±1.39)pg/mL,which were significantly higher than 1.56±0.31,(11.52±2.20)%,(94.57±3.29)pg/mL,(42.37±1.28)pg/mL in healthy group;the levels of PLT,Treg,Th2,and serum IL-4,IL-10 were(45.62±18.55)×10^(9)/L,(3.60±0.75)%,(1.26±0.51)%,(24.92±1.05)pg/mL,(141.28±1.67)pg/mL,which were significantly lower than(198.73±25.21)×10^(9)/L,(3.98±0.37)%,(1.72±0.24)%,(42.37±1.72)pg/mL,(196.03±0.25)pg/mL in healthy group;the differences were statistically significant(P<0.05).Correlation analysis showed that the relative expression of microRNA-155 in PBMC was negatively correlated with PLT(r=-0.683),IL-4(r=-0.611),IL-10(r=-0.520),Treg(r=-0.522),and Th2(r=-0.572),with P<0.05,and there was a positive correlation with IFN-γ(r=0.634),IL-2(r=0.545),and Th1(r=0.821),with P<0.05.Conclusion MicroRNA-155 is highly expressed in PBMC of ITP patients and has a certain correlation with PLT,IL-4,IL-10,Treg,IFN-γ,IL-2,Th1,and Th2.It has the potential to be a therapeutic target for ITP patients.

关 键 词：原发性免疫性血小板减少症 MicroRNA-155 外周血单个核细胞 表达 相关性 

分 类 号：R725.5[医药卫生—儿科]