Antagonistic interaction between male-killing and cytoplasmic incompatibility induced by Cardinium and Wolbachia in the whitefly,Bemisia tabaci  

作　　者：Ning Lv Jing Peng Xin-Yi Chen Chang-Fei Guo Wen Sang Xing-Min Wang Muhammad ZAhmed Yong-Yu Xu Bao-Li Qiu 

机构地区：[1]Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province,South China Agricultural University,Guangzhou,China [2]Engineering Research Center of Biocontrol,Ministry of Education,Guangzhou,China [3]Maoming Branch,Guangdong Laboratory of Lingnan Modern Agriculture,Maoming,Guangdong Province,China [4]Florida Department of Agriculture and Consumer Services,1911 SW 34th Street,Gainesville,USA [5]College of Plant Protection,Shandong Agricultural University,Taian,Shandong Province,China

出　　处：《Insect Science》2021年第2期330-346,共17页昆虫科学（英文版）

基　　金：The authors thank Kerry M.Oliver and David Plotkin for critical reviews of this manuscript,and we thank Pei-Qiong Shi for culture of whitefly lines.This work was supported by the NSFC-Guangdong Joint Research Fund(U1701231);the National Natural Science Foundation of China(31672028);Key-Area Research and Development Program of Guangdong Province(2018B020205003)to BLQ.The funders had no role in the study design,data collection and interpretation,or the decision to submit the work for publication.

摘　　要：Cardinium and Wolbachia are maternally inherited bacterial symbionts of arthropods that can manipulate host reproduction by increasing the fitness of infected females.Here,we report that Cardinium and Wolbachia coinfection induced male-killing and cytoplasmic incompatibility(CI)when they coexisted in a cryptic species of whitefly,Bemisia tabaci Asia II7.Cardinium and Wolbachia symbionts were either singly or simul-taneously localized in the bacteriocytes placed in the abdomen of B.tabaci nymphs and adults.Cardinium-Wolbachia coinfection induced male-killing and resulted in a higher female sex ratio in the intraspecific amphigenetic progeny of Asia II7 IcwH and IcwL lines;interestingly,male-illing induction was enhanced with increased Cardinium titer.Moreover,single infection of Wolbachia induced partial CI in the Asia II7 Iw line and resulted in reduced fecundity,higher embryonic mortality,and lower female sex ratio.The uninfected Asia II7 Iu line had significantly higher fecundity,lower embryonic and nymphal mortalities,and a lower level of CI than both the Wolbachia infected Asia II7 Iw line and the Cardinium--Wolbachia-coinfected Asia II7 IcwH line.Our findings indicate that Cardinium-Wolbachia coinfection induced male-killing,which may have had antag-onistic effects on Wolbachia-induced CI in the Asia II7 whiteflies.For the first time,our study revealed that B.tabaci Asia II7 reproduction is co-manipulated by Cardinium and Wolbachia endosymbionts.

关 键 词：antagonistic interaction Bemisia tabaci COINFECTION ENDOSYMBIONT repro-ductive manipulation sex ratio 

分 类 号：S435[农业科学—农业昆虫与害虫防治]