Wolbachia enhances expression of NICYP4CE1 in Nilaparvata lugens in response to imidacloprid stress  

作　　者：Tingwei Cai Yunhua Zhang Yu Liu Xiaoqian Deng Shun He Jianhong Li Hu Wan 

机构地区：[1]Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory,College of Plant Science and Technology,Huazhong Agricultural University,Wuhan,China

出　　处：《Insect Science》2021年第2期355-362,共8页昆虫科学（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China(31871991);the Natural Science Foundation of Hubei Province(2019CFB471);the Fundamental Research Funds for the Central Universities(2662018JC049).

摘　　要：The brown planthopper,Nilaparvata lugens,is one of the main insect pests of rice.The N.lugens gene NICYP4CE1 encodes cytochrome P450 monooxygenase(P450),which is a key enzyme in the metabolism of the insecticide imidacloprid.Previous research has suggested that the expression of NICYP4CEI is induced by imidacloprid stress,but the effect of bacterial symbionts on its expression has not been determined.The results of this study show that exposure to subtoxic imidacloprid changed the structure of the bacterial symbiont community in N.lugens.Specifically,the total bacterial content increased but the bacterial species diversity significantly decreased.Wolbachia accounted for the largest proportion of bacteria in N.lugens;its abundance significantly increased after subtoxic imidacloprid exposure.The transcript level of NICYP4CEI was significantly increased by imidacloprid,but this effect was significantly weakened after Wolbachia was cleared with tetracycline.This result suggests that Wolbachia enhances the expression of N1CYP4CE1 to promote the detoxification metabolic response to imidacloprid stress.Understanding the effect of bacterial symbionts on gene expression in the host provides a new perspective on interactions between insecticides and their target insect pests,and highlights that subtoxic imidacloprid exposure may raise the risk of insecticide resistance by altering the structure of bacterial symbiont communities.

关 键 词：host-microbe interaction IMIDACLOPRID NICYP4CE1 P450 WOLBACHIA 

分 类 号：S435.112[农业科学—农业昆虫与害虫防治]