Diversity of bacteria in different life stages and their impact on the development and reproduction of Zeugodacus tau(Diptera:Tephritidae)  

作　　者：MdShibly Noman Ge Shi Li-Jun Liu Zhi-Hong Li 

机构地区：[1]Department of Entomology,MOA Key Lab of Pest Monitoring and Green Management,College of Plant Protection,China Agricultural University,Beijing,China

出　　处：《Insect Science》2021年第2期363-376,共14页昆虫科学（英文版）

基　　金：We thank the other members of Plant Quarantine and Invasion Biology Laboratory,China Agricultural University(CAUPQL)who helped us during this study.The research was supported by the National Natural Science Foundation Project of China(31801802);Fundamental Research Funds for the Central Universities,China(2019 TC060).

摘　　要：Fruit flies usually harbor diverse communities of bacteria in their digestive systems,which are known to play a significant role in their fitness.However,little information is available on Zeugodacus tau,a polyphagous pest worldwide.This study reports the first extensive analysis of bacterial communities in different life stages and their effect on the development and reproduction of laboratory-reared Z tan.Cultured bacteria were identified using the conventional method and all bacteria were identified by highthroughput technologies(16S ribosomal RNA gene sequencing of V3-V4 region).A total of six bacterial phyla were identified in larvae,pupae,and male and female adult flies,which were distributed into 14 classes,32 orders,58 families and 96 genera.Proteobacteria was the most represented phylum in all the stages except larvae.Enterobacter,Klebsiella,Providencia,and Pseudomonas were identified by conventional and next-generation sequencing analysis in both male and female adult flies,and Enterobacter was found to be the main genus.After being fed with antibiotics from the first instar larvae,bacterial diversity changed markedly in the adult stage.Untreated flies laid eggs and needed 20 days before oviposition while the treated flies showed ovary development inhibited and were not able to lay eggs,probably due to the alteration of the microbiota.These findings provide the cornerstone for unexplored research on bacterial function in Z tau,which will help to develop an environmentally friendly management technique for this kind of harmful insect.

关 键 词：ANTIBIOTICS bacterial diversity FITNESS OVIPOSITION symbiotic bacteria Zeugodacus tau 

分 类 号：Q96[生物学—昆虫学]