A microbiological assay for biotin determination in insects  

作　　者：Fei-Rong Ren Bing Bai Ji-Sheng Hong Yan-Zhen Huang Jun-Bo Luan 

机构地区：[1]Liaoning Key Laboratory of Economic and Applied Entomology,College of Plant Protection,Shenyang Agricultural University,Shenyang,China [2]College of Plant Protection,Shenyang Agricultural University,Shenyang,China

出　　处：《Insect Science》2021年第2期415-418,共4页昆虫科学（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China(Project 31871967);High-tech R&D Program of Liaoning(Project 2019JH2/10200012);High-Level Talent Support Foundation from Liaoning,Shenyang and Shenyang Agricultural University(Project XLYC1902104,RC180025 and 880418001)and Natural Science Foundation of Liaoning(20180550214);The authors thank Professor Shu-Sheng Liu for providing us with the whitefly B.tabaci MEAM1 culture,and thank Professor Chuan-Xi Zhang for providing us with the brown planthopper N.lugens culture,Dr.Yu-Ting Li,Yan-Bin Wang,Xiao-Rui Xu for help with experiments.

摘　　要：Dear Editor,As the coenzyme of multiple carboxylases,biotin plays key roles in intermediary metabolism and animal nutrition(Listed 1990;Douglas,2017).Therefore,the analytical determination of biotin levels in diverse materials is a critical aspect of B-vitamin research.Microbiological assays are classic methods of measuring B-vitamin levels.Briefly,the growth of B-vitamin-dependent microorganisms is proportional to the amount of the specific B-vitamin in the medium,and the growth can be measured by assessing the change in the medium's turbidity.

关 键 词：DETERMINATION VITAMIN CRITICAL 

分 类 号：Q96[生物学—昆虫学]