Phospholipase C gamma(PLCγ)regulates soluble trehalase in the 20E-induced fecundity of Apolygus lucorum  

作　　者：Yong-An Tan Xu-Dong Zhao Hou-Jun Sun Jing Zhao Liu-Bin Xiao De-Jun Hao Yi-Pin Jiang 

机构地区：[1]nstitute of Plant Protection,Jiangsu Academy of Agricultural Sciences,Nanjing,China [2]College of Forestry.Nanjing Forestry University,Nanjing,China [3]Jiangsu Xuzhou Sweet Potato Research Center.Xuzhou,Jiangsu Province,China

出　　处：《Insect Science》2021年第2期430-444,共15页昆虫科学（英文版）

基　　金：This study was supported by Chinese Agricultural Research System(CARS-15-18),the Integration Research and Demonstration of the Technology of Cotton Fertilizer and Pesticide Reduction of China(2017YFD0201900);Jiangsu Agricultural Science and Technology Innovation Fund(CX(19)3098);grants from the National Natural Science Fund of China(31301668);JAAS Research Foundation(6111613).

摘　　要：Apolygus lucorum is the dominant pathogenic insect attacking Bacillus thuringiensis(Bt)cotton in China.Additionally,20-hydroxyecdysone(20E)has important functions in many biological processes,including insect reproduction.Phospholipase C(PLC),which is an essential enzyme for phosphoinositide metabolism,is involved in 20E signal transduction,but its function in 20E-mediated reproduction in A.lucorum remains unclear.In this study,20E increased A/PLCγ transcription as well as the abundance and activity of the encoded protein during molting and metamorphosis.The 20E treatment also induced the considerable accumulation of two second messengers,inositol triphosphate and diacylglycerol.The expression levels of genes encoding vitellogenin(AlVg)and soluble trehalase(AlTre-1)were similar to those of AlPLCy,and were upregulated in response to 20C.The silencing of AlPLCγ resulted in downregulated expression o f AITre-γ smdAlVg.However,the silencing of AlTre-1 and AlVg did not affect AlPLCγ expression.Moreover,the silencing of AlVg did not alter AlTre-1 expression.Furthermore,an examination of the insect specimens indicated that AlPLCy is required for female adult reproduction,and that downregulated expression of this gene is associated with decreases in fecundity,adult longevity,and egg hatching rate as well as delayed oocyte maturation.We propose that 20E regulates AlTre-1 expression via AlPLCy and affects Vg expression as well as ovary development to facilitate the reproductive activities of A.lucorum females.

关 键 词：Apolygus luconmn phospholipase C REPRODUCTION soluble trehalase 20-HYDROXYECDYSONE VITELLOGENIN 

分 类 号：Q96[生物学—昆虫学]