Functional characterization of pheromone receptor candidates in codling moth Cydia pomonella(Lepidoptera:Tortricidae)  

作　　者：Ke Tian Wei Liu Li-Kai Feng Tian-Yu Huang Gui-Rong Wang Ke-Jian Lin 

机构地区：[1]State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing,China [2]Institute of Plant Protection,Xinjiang Academy of Agricultural and Reclamation Science,Shihezi,Xinjiang Uygur Autonomous Region,China [3]Lingnan Guangdong Laboratory of Modern Agriculture,Genome Analysis Laboratory of the Ministry of Agriculture,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen,Guangdong Province,China

出　　处：《Insect Science》2021年第2期445-456,共12页昆虫科学（英文版）

基　　金：This work was supported by the National Natural Science Foundation of China(31672033);China postdoctoral science funding(2017M611069).We thank Mr.Song Cao,Mr.Yipeng Liu,Mr.Shuai Liu and Ms.Sai Zhang for help with the molecular biology experiments.

摘　　要：Sex pheromones serve a critical role in Lepidopterans finding mates.Male moths perceive and react to sex pheromones emitted by conspecific females through a delicate pheromone communication system.Pheromone receptors(PRs)are the key sensory elements at the beginning of that process.The codling moth(Cydia ponwonella)is an important pome fruit pest globally and a serious invasive species in China.Pheromonebased techniques have been used successfully in monitoring and controlling this species.We conducted ribonucleic acid sequencing analysis of the codling moth antennal transcriptome and identified 66 odorant receptors(ORs)in a population from Xinjiang province,China,of which 14 were PRs,including two novel PRs(CpomOR2e and CpomOR73).Four PRs that contain full-length open reading frames(CpomORl,OR2a,OR5,OR7)and four PRs with ligands that have not been reported previously(CpomORl,OR2a,OR5,OR7)were selected to deorphanize in the heterologous Xenopus oocyte expression system.Specifically,we found that CpomOR2a and CpomOR5 responded to(£,£)-8,10-dodecadien-l-yl acetate(codlemone acetate).Furthermore,CpomOR5(EC50=1.379 x 10-8 mol/L)was much more sensitive to codlemone acetate than CpomOR2a(EC50=1.663×10^(-6) mol/L).Since codlemone acetate is an important component of C.pomonella sex pheromone,our results improve the current understanding of pheromone communication in codling moths and will be helpful for the development of pest management strategies.

关 键 词：antennal transcriptome codlemone acetate Cydia pomonella' pheromone receptor 

分 类 号：S433[农业科学—农业昆虫与害虫防治]