Oral delivery of dsHvlwr is a feasible method for managing the pest Henosepilachna vigintioctopunctata(Coleoptera:Coccinellidae)  

作　　者：Jing Lu Zhuo-Qi Liu Wei Guo Mu-Juan Guo Shi-Min Chen Chun-Xiao Yang You-Jun Zhang Hui-Peng Pan 

机构地区：[1]Key Laboratory of Bio-Pesticide Innovation and Application of Guangdong Province,South China Agricultural University,Guangzhou,China [2]Ling nan Guangdong Laboratory of Modern Agriculture,Guangzhou,China [3]Department of Plant Protection,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing,China

出　　处：《Insect Science》2021年第2期509-520,共12页昆虫科学（英文版）

基　　金：Special thanks to Dr.Xuguo Zhou(University of Kentucky)for his valuable suggestion on preparing this manuscript.This research was supported by the National Key R&D Program of China(2017YFD0200900);National Natural Science Foundation of China(31972269),and GDUPS(2017).The granting agencies had no role in the study design,data collection and analysis,decision to publish,or preparation of the manuscript.

摘　　要：RNA interference(RNAi)techniques have emerged as powerful tools that facilitate development of novel management strategies for insect pests,such as Henosepilachna vigintioctopunctata(Coleoptera:Coccinellidae),which is a major pest of solanaceous plants in Asia.In this study,the potential of oral delivery of in vvYro-synthesized and bacterially expressed double-stranded H.vigintioctopunctata lesswright(Iwr)gene(dsHvlwr)to manage of H.vigintioctopunctata was investigated.Our results showed that the gene Hvlwr had a 480-bp open reading frame and encoded a 160-amino acid protein.Hvlwr expression levels were greater in the fat body than other tissue types.Hvlwr silencing led to greater H.vigintioctopunctata mortality rates and appeared to be time-and partially dose-dependent,likely as a result of the number of hemocytes increasing with dsRNA concentration,but decreasing with time.Bacterially expressed dsHvlwr that was applied to leaf discs caused 88%,66%,and 36%mortality in 1st instars,3rd instars,and adults after 10,10,and 14 d,respectively;when applied to living plants,there was greater mortality in 1 st and 3rd instars,but there was no effect on adults.Furthermore,dsHvlwr led to improved plant protection against H.vigintioctopunctata.Our study shows an effective dietary RNAi response in H.vigintioctopunctata and that Hvlwr is a promising RNAi target gene for control of this pest species.

关 键 词：bacterially expressed dsRNA HEMOCYTES Henosepilachna vigintioctopunctata lesswright:RNAi 

分 类 号：S433.5[农业科学—农业昆虫与害虫防治]