混合菌协同降解煤泥水中聚丙烯酰胺的试验研究  

Experimental study on co-degradation of polyacrylamide in coal slime water by mixed bacteria

作　　者：张东晨[1] 王方略 王涛[1] 戴雯[1] ZHANG Dongchen;WANG Fanglue;WANG Tao;DAI Wen(School of Material and Engineering,Anhui University of Science and Technology,Huainan 232001,China)

机构地区：[1]安徽理工大学材料科学与工程学院,安徽淮南232001

出　　处：《洁净煤技术》2021年第1期209-216,共8页Clean Coal Technology

基　　金：国家自然科学基金资助项目(51274012)。

摘　　要：为解决煤泥水中残留聚丙烯酰胺降低浮选效果和污染环境等问题,研究了黄孢原毛平革菌与球红假单胞菌等体积混合降解聚丙烯酰胺时的环境条件和营养物质利用情况,同时采用红外光谱和黏度法分析降解产物,并对降解液进行酶活测试分析。结果表明,黄孢原毛平革菌和球红假单胞菌单独添加时对聚丙烯酰胺的降解率分别为30.2%和24.9%,混合菌降解率为34.3%。在最佳条件下,混合菌对聚丙烯酰胺降解率达到37.5%。在不同条件下降解效果明显不同,添加少量的葡萄糖和酒石酸铵可以促进降解;且温度对聚丙烯酰胺降解影响显著。降解后的聚丙烯酰胺分子结构的侧链酰胺基水解成羧基,作为微生物所需的氮源;在强氧化酶作用下,碳链骨架打断,导致分子量下降,提供微生物所需的碳源;降解液中检测出漆酶(Lac)、锰过氧化物酶(Mn P)和木质素过氧化物酶(Li P),酶活分别为16.48、12.09和6.73 U/m L。In order to solve the problems of residual polyacrylamide(PAM)in the coal slime water,such as reducing the flotation performance and polluting the environment.Firstly,the mixed strain was formed by combining Phanerochaete chrysosporium(P.chrysosporium)and Rhodopseudomonas spheroides(R.spheroides)in equal volume.The effects of environmental factors and nutritional conditions on PAM degradation were investigated by mixed strain.Secondly,the degradation products were analyzed by FT-IR and viscosity method.Finally,the enzyme activity of degradation culture was tested.The results indicate that P.chrysosporium and R.spheroides can degrade PAM with degradation rate of 30.2%and 24.9%,respectively when added separately.The mixed strain degradation rate of PAM reaches 34.3%.The degradation rate of PAM reaches 37.5%under the optimal conditions.Different conditions will lead to significantly different degradation effects.A small amount of the glucose and the ammonium tartrate can promote the degradation,and the temperature has a significant effect on the degradation of PAM.The amide group of the side chain of PAM is converted into carboxyl group,which can be used as nitrogen source for microorganisms.The carbon chain skeleton of PAM is broken under the action of strong oxidase,resulting in the decline of molecular weight,which can provide carbon source for microbes.Laccase,manganese peroxidase and lignin peroxidase are detected in the degraded fermantation liquid with enzyme activity of 16.48 U/mL,12.09 U/mL and 6.73 U/mL,respectively.

关 键 词：煤泥水 球红假单胞菌 黄孢原毛平革菌 聚丙烯酰胺 生物降解 

分 类 号：TD926.2[矿业工程—选矿]