HF-LPME与HPLC联用测定槲皮素LogD和富集倍数  

Hollow fiber liquid-phase microextraction coupled with HPLC for determination of Quercetin LogD and enrichment factor

作　　者：宝贵荣 通拉嘎[1] 李优鑫 包建民[1] BAO Gui-rong;TONG La-ga;LI You-xin;BAO Jian-min(College of Chemistry and Environment,Hohhot Minzu College,Hohhot 010050,China;School of Pharmaceutical Science and Technology,Tianjin University,Tianjin 300072,China)

机构地区：[1]呼和浩特民族学院化学与环境学院,内蒙古呼和浩特010050 [2]天津大学药物科学与技术学院,天津300072

出　　处：《现代化工》2021年第2期265-269,共5页Modern Chemical Industry

基　　金：内蒙古自然科学基金项目(2018MS02007);呼和浩特市科技局项目(2019-农-13)。

摘　　要：建立了96-孔板中空纤维膜液相微萃取(HF-LPME)与高效液相色谱(HPLC)联用技术测定槲皮素油水分配系数和不同介质中富集倍数的方法。对96-孔板中空纤维膜液相微萃取条件进行优化,优化后的萃取条件为:60%聚丙烯中空纤维膜,萃取溶剂为正辛醇,振荡速度为173 r/min,萃取温度为室温,样品溶液的pH为4.0,萃取时间为90 min。槲皮素在浓度范围内线性关系良好,相关系数r2≥0.992 3,加样回收率≥93.12%。槲皮素油水分配系数为2.05,在水溶液中的富集倍数高于血清样品中的富集倍数,而血清样品中加入2%甲醇时富集倍数最大。血清样品中加入2%甲醇时槲皮素富集倍数为99.54,是在水溶液中富集倍数156.01的63.8%。该技术快速、简便,灵敏度高,具有广泛的应用前景。A 96-well based hollow fiber liquid phase microextraction( HF-LPME) coupled with HPLC method is developed to determine the oil-water partition coefficient and enrichment factor of quercetin in different media.Conditions for 96-well hollow fiber liquid phase microextraction are optimized as follows: polypropylene hollow fiber has a porosity of60%,octanol is used as extraction solvent,p H of sample solution is 4,shaking speed is 173 r·min-1,and the extraction performs for 90 min at room temperature.Calibration curves of quercetin show good linear relationship in the concentration range of 0. 921-15 mg·L-1,with a recovery not less than 93. 12%.The water-oil partition coefficient of quercetin is 2. 05.The enrichment factor of quercetin in water is higher than that in serum samples.When 2% methanol is added into serum samples,the enrichment factor of quercetin is 99. 54,reaching the highest,which is equivalent to 63. 8% of the 156. 01 of quercetin in pure water. In conclusion,this method has a wide application prospect due to low cost,easy,and high sensitivity.

关 键 词：中空纤维膜液相微萃取 高效液相色谱 槲皮素 

分 类 号：R917[医药卫生—药物分析学]