动脉粥样硬化兔血清、外周血单核细胞、主动脉组织B7-H3、B7-H4表达变化及意义  

Expression changes and significance of B7-H3 and B7-H4 in serum,PBMC,and aortic tissues of rabbits with atherosclerosis

作　　者：李志乐[1] 韦秋宇 言纬 刘莉[1] 黄照河[1] 韦宝敏[1] LI Zhile;WEI Qiuyu;YAN Wei;LIU Li;HUANG Zhaohe;WEI Baomin(Affiliated Hospital of Youjiang Medical University for Nationalities,Baise 533000,China)

机构地区：[1]右江民族医学院附属医院,广西百色533000

出　　处：《山东医药》2021年第11期37-40,44,共5页Shandong Medical Journal

基　　金：广西卫计委医药卫生项目(Z2015097);广西高校中青年教师基础能力提升项目(2018KY0453)。

摘　　要：目的观察动脉粥样硬化(AS)兔血清、外周血单核细胞、主动脉组织中协同刺激分子B7-H3、B7-H4表达变化,并探讨其可能的机制。方法选取成年雄性新西兰大白兔20只,随机分为AS组和对照组各10只。AS组持续给予高脂饮食14周,对照组给予普通饮食14周。分组处理前(0周)和分组处理14周末,比较两组血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)及白细胞介素1β(IL-1β)、γ-干扰素(IFN-γ)、白细胞介素10(IL-10)。两组分组处理14周末,采用HE染色观察主动脉形态及病理情况,记录斑块面积比及内膜厚度,采用实时荧光定量PCR法检测外周血单核细胞与主动脉组织B7-H3、B7-H4 mRNA表达。结果 AS组14周时兔血清TC、TG、LDL-C、IL-1β、IFN-γ水平均高于同组0周及对照组同时间点,HDL-C、IL-10、sB7-H3、sB7-H4水平均低于同组0周及对照组同时间点(P均<0.05)。主动脉形态及病理结果显示,对照组兔主动脉内膜光滑完整;AS组兔主动脉呈弥漫性改变,可见脂样物质突出管腔,内膜增生增厚且可见泡沫细胞与脂质沉积,中膜平滑肌细胞增生。AS组兔斑块面积比为66.82%±15.45%、内膜厚度(86.02±12.32)μm,对照组分别为0、(8.12±3.85)μm;两组比较P均<0.05。与对照组比较,AS组兔外周血单核细胞与主动脉组织B7-H3、B7-H4mRNA表达均降低(P均<0.05)。血清sB7-H3、sB7-H4与IL-1β、IFN-γ均呈负相关关系,与IL-10均呈正相关关系(P均<0.05)。结论 AS兔血清、外周血单核细胞、主动脉组织中协同刺激分子B7-H3、B7-H4表达均降低,B7-H3、B7-H4可能通过促进IL-10分泌及抑制IL-1β、IFN-γ表达而减轻AS的炎症反应。Objective To observe the expression changes of B7-H3 and B7-H4 in serum,peripheral blood mononu⁃clear cells(PBMCs),and aortic tissues of rabbits with atherosclerosis(AS)and to explore its possible mechanism.Methods Twenty adult male New Zealand rabbits were randomly divided into the AS group and the control group(n=10).The rabbits in the AS group were given high-fat diet for 14 weeks,while the rabbits in the control group were given normal diet for 14 weeks.Before treatment(0 week)and 14 weeks after treatment,serum total cholesterol(TC),triglyceride(TG),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),interleukin 1β(IL-1β),γ-interferon(IFN-γ),and interleukin 10(IL-10)were compared between the two groups.After 14 weeks of treat⁃ment,the aorta morphology and pathology were observed by HE staining,plaque area ratio and intimal thickness were re⁃corded,and the expression levels of B7-H3 and B7-H4 in the aortic tissues and PBMCs were detected by real-time fluores⁃cence quantitative PCR.Results The serum levels of TC,TG,LDL-C,IL-1β,and IFN-γof rabbits in AS group at 14 weeks were higher than those at 0 week and the control group at the same time point,but the levels of HDL-C,IL-10,sB7-H3,and sB7-H4 were lower than those at 0 week and the control group at the same time point(all P<0.05).Morphology and pathology of the rabbit aorta in the two groups showed that the aorta intima in the control group was smooth and complete,while the aorta of the rabbits in the AS group presented diffuse changes,with prominent lumen of lipid-like material,thickened intima hyperplasia,foam cells and lipid deposition,and proliferation of smooth muscle cells in the media.The plaque area ratio and intima thickness of the rabbits in AS group were 66.82%±15.45%and(86.02±12.32)μm,compared with 0,(8.12±3.85)μm in control group(all P<0.05).Compared with the control group,the expression levels of B7-H3 and B7-H4 mRNA in peripheral blood monocytes and aortic tissues of rabbits in AS group decre

关 键 词：动脉粥样硬化 B7-H3 B7-H4 细胞因子 炎症反应 兔 

分 类 号：R543.5[医药卫生—心血管疾病]