基于电喷雾质谱法及化学计量法筛选鹿茸促睾丸间质细胞分泌睾酮的活性成分  

The Active Components of Testosterone Secreted by Antler Testis Stromal Cells Rapidly Screened by Electrospray Tandem Mass Spectrometry and Chemometrics

作　　者：王亚苹 李晶峰 张凯月 张楠茜 高颖[1] 张辉[3] 孙佳明[3] WANG Ya-ping;LI Jing-feng;ZHANG Kai-yue;ZHANG Nan-xi;GAO Ying;ZHANG Hui;SUN Jia-ming(College of Pharmacy,Changchun University of Chinese Medicine,Changchun 130117,China;Northeast Asia Biotechnology Co.Ltd.,Changchun 130117,China;Jinlin Ginseng Academy,Changchun University of Chinese Medicine,Changchun 130117,China)

机构地区：[1]长春中医药大学药学院,吉林长春130117 [2]东北亚生物科技有限公司,吉林长春130117 [3]长春中医药大学人参科学研究院,吉林长春130117

出　　处：《现代食品科技》2021年第4期262-268,233,共8页Modern Food Science and Technology

基　　金：国家自然科学基金面上项目(81373936);吉林省科技厅重点研发项目(20200404081YY);吉林省卫生与健康青年科技骨干培养计划项目(2020Q026)。

摘　　要：采用电喷雾串联质谱法(ESI-MS/MS)及化学计量法快速筛选鹿茸促睾丸间质细胞分泌睾酮的活性成分。运用电喷雾串联质谱法分别检测6批鹿茸鲜品和6批鹿茸传统加工品中化学成分,并测定各样品增加大鼠睾丸间质细胞的睾酮分泌量,联合主成分分析(PCA)、偏最小二乘法判别分析(PLS-DA)和正交偏最小二乘法判别分析(OPLS-DA)对得出的一级质谱数据进行分析;实现鹿茸鲜品和鹿茸传统加工品两组样品的快速区分。采用偏最小二乘法(PLS)和灰色关联度分析(GRA)筛选出其中具有潜在促睾丸间质细胞分泌睾酮的活性成分,通过对二级质谱数据分析明确其中的可能活性成分的结构和组成。结果表明,鹿茸鲜品与加工品的组成成分有显著差异,且二者均具有良好的促睾丸间质细胞分泌睾酮的活性,其中活性较强的成分主要为Tyr-Ser-Phe(m/z416)和Phe-Phe-Leu/Ile(m/z 426)。本方法能够简单快速分析鹿茸中促睾丸间质细胞的活性成分,为鹿茸作为保健食品的后续开发提供参考。Electrospray tandem mass spectrometry(ESI-MS/MS)and chemometrics were used to screen the active components of testosterone secreted by testis stromal cells of Antler antler.Using electrospray tandem mass spectrometry,the chemical composition of 6 batch of pilose antler and 6 batch of velvet antler prepared by traditional process was detected.The testosterone secretion of rat leydig cells was also determinated.The principal component analysis(PCA),partial least squares discriminant analysis(PLS-DA)and orthogonal partial least squares discriminant analysis(OPLS-DA)were used to analyze the mass spectrometry data.Rapid differentiation of fresh velvet antler and traditional processed products was achieved.Partial least square method(PLS)and gray correlation degree analysis(GRA)were used to select the active components with potential testosterone secretion by testicular stromal cells.The structure and composition of the possible active components were determined by secondary mass spectrometry data analysis.The results showed that the components of fresh antler and traditional processed products were significantly different,and both of them had good activity of testosterone secretion by testicular stromal cells.The most active components were Tyr-Ser-Phe(m/z 416),PHE-Phe-Leu/Ile(m/z 426),respectively.This method could be used to analyze the active components of testicular stromal cells in antler and provide reference for the development of antler as health food.

关 键 词：鹿茸 电喷雾串联质谱 化学计量法 睾丸间质细胞 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程] O657.63[轻工技术与工程—食品科学与工程]