激光共聚焦扫描显微系统在心血管支架表面内皮化分析研究中的应用  

Application of laser scanning confocal microscopy(LSCM)system in re-endothelialization analysis of the cardiovascular stents surface

作　　者：谭星 涂秋芬[1,2] 郑晓柯 胡张梅 TAN Xing;TU Qiu-fen;ZHENG Xiao-ke;HU Zhang-mei(School of Materials Science and Engineering,Southwest Jiaotong University,Chengdu Sichuan 610030;Analysis and Testing Center,Southwest Jiaotong University,Chengdu Sichuan 610030,China)

机构地区：[1]西南交通大学材料科学与工程学院,四川成都610030 [2]西南交通大学分析测试中心,四川成都610030

出　　处：《电子显微学报》2021年第2期163-169,共7页Journal of Chinese Electron Microscopy Society

基　　金：国家自然科学基金-面上资助项目(No.31570957)。

摘　　要：目的:观察内皮细胞(ECs)在植入的心血管支架表面的分布与生长状态,探讨支架材料表面的内皮化情况,并对支架表面内皮化程度的影响因素进行了初步探究。方法:在对内皮细胞不同结构进行特异性染色标记的情况下,利用激光共聚焦扫描显微系统连续扫描观察荧光标记的内皮细胞在植入体内一个月后的支架材料表面的表达程度。结果:在无任何促内皮分子或药物情况下,支架材料本身分布的内皮细胞较为稀疏,整体内皮化程度不高;而在材料本身涂覆促内皮分子或药物的情况下,支架材料表面内皮细胞分布较为紧密,且规则有序,其整体内皮化程度更高。结论:利用激光共聚焦扫描系统能够从宏观和微观角度上有效实现支架材料表面内皮化程度的定性定量评价。Objective:To observe the distribution and growth status of endothelial cells(ECs)on the surface of cardiovascular stents,to investigate the endothelialization of the surface of the stents and to explore the factors affecting the degree of endothelialization on the surface of the stent.Methods:In the case of specific staining and labeling of different structures of endothelial cells the laser confocal scanning microscopy(LSCM)system was used to continuously scan and observe the fluorescently labeled endothelial cells on the surface of the stents which implanted in vivo after one month.Results:In the absence of any endothelial molecules or drugs,the endothelial cells distributed on the stent materials were relatively sparse,and the degree of endothelialization was not enough.However,endothelial cells are distributed tightly and in a regular and orderly manner on the surface of the stents which were coated with endothelial molecules or drugs.In addition,it has a higher degree of endothelialization after one week.Conclusion:The laser confocal scanning system can effectively realize the qualitative and quantitative evaluation of the surface endothelialization degree of the stent material from the micro and macro perspectives.

关 键 词：激光共聚焦扫描系统 内皮细胞 心血管支架 表面内皮化 

分 类 号：Q336[生物学—遗传学] R331[医药卫生—人体生理学]