拔罐对大鼠环跳穴区皮肤组织相关神经肽及化学物质表达变化的影响  

Effect of Baguan on the Expression of Neuropeptides and Chemical Substances in Skin Tissue of“Huantiao”(GB30)Acupoint Area

作　　者：刘惠 徐东升[2] 陈虹[3] 王莹莹[2] 杨金生[1] LIU Hui;XU Dongsheng;CHEN Hong;WANG Yingying;YANG Jinsheng(Shaanxi University of Chinese Medicine,Xianyang 712046,China;Institute of Acupuncture and Moxibustion,China Academy of Chinese Medical Sciences,Beijing 100700,China;The Second Affiliated Hospital of Shaanxi University of Chinese Medicine,Xianyang 712046,China)

机构地区：[1]陕西中医药大学,咸阳712046 [2]中国中医科学院针灸研究所,北京100700 [3]陕西中医药大学第二附属医院,咸阳712046

出　　处：《世界中医药》2021年第5期769-774,共6页World Chinese Medicine

基　　金：国家自然科学基金项目(81674079);北京市自然科学基金项目(7172149)。

摘　　要：目的:通过使用免疫组化和共聚焦技术研究拔罐对环跳穴区皮肤组织肥大细胞(Mast Cell)、神经肽Y(Neuropeptide Y,NPY)及5-羟色胺(5-Hydroxytryptamine,5-HT)的影响。方法:选取10只健康SD大鼠,剃除双后肢环跳穴区毛发,充分暴露穴区皮肤组织,在左侧环跳穴进行拔罐干预,拔罐后即刻灌流,并分别取正常大鼠和拔罐大鼠左侧穴区皮肤组织,长宽均为1 cm。使用25%高渗糖脱水后用恒冷箱切片机制成20μm厚的组织切片,然后分别用Mast Cell、NPY、5-HT抗体进行荧光免疫组化检测。结果:与正常组比较,拔罐组大鼠环跳穴区皮肤组织表皮层明显增厚,且结构不清,真皮及皮下组织血管扩张挤压变形,Mast Cell、NPY、5-HT阳性表达明显增强。Mast Cell和5-HT在表皮和真皮内靠近血管和毛囊聚集,在皮下组织中大量散在分布围绕血管形成不连续的细胞带。NPY神经纤维在真皮及皮下组织的分布以串珠形式为主,在真皮下层绕血管毛囊汇聚明显。使用荧光显微镜分别在10倍镜下对5-HT和Mast Cell阳性细胞进行计数,在20倍显微镜下NPY阳性表达纤维的长度进行统计,结果表明拔罐观察组各项指标均高于正常组,2组比较,差异有统计学意义(P<0.05)。结论:拔罐能够使环跳穴区皮肤组织中Mast Cell、NPY、5-HT的表达上调,这些神经活性物质的释放可能激活相关信号传递通路而实现拔罐的效果。Objective:To investigate the influence of baguan on mast cell neuropeptide Y(NPY),5-Hydroxytryptamine(5-HT)in skin tissue at Huantiao(GB30)by using immunohistochemistry combined with confocal technique.Methods:A total of 10 rat was used in this experiment.Their skin tissue on hind limbs Huantiao(GB30)was exposed by shaving.Subsequently,baguan was carried out on the left side of Huantiao(GB30).After baguan,the local skin tissue of 1 cm×1 cm on bilateral Huantiao(GB30)of rats were collected,and tissue sections of 20μm thickness were prepared by dehydration with 25%hypertonic sugar and sectioning in constant cooling box The Mast Cell、NPY、5-HT antibodies were used for fluorescent immunohistochemical test.Results:Compared with the normal group,the epidermal layer of the skin significantly thickened,with unclear structure,dermis and subcutaneous tissues dilated and extruded,and the positive expressions of Mast Cell,NPY and 5-HT increased significantly.Mast Cell and 5-HT aggregated in close proximity to the blood vessels and hair follicles in intra-epidermis and dermis,and are widely dispersed in subcutaneous tissue layer forming a discrete band of cells surrounding the blood vessels.NPY is distributed in the dermis and subcutaneous tissues in the beads form,and aggregated in close proximity to the blood vessels in the subdermal layer significantly.The markers of 5-HT and Mast Cell positive cells were counted under 10 times microscope,and the length of NPY positive fibers was counted under 20 times of microscope.The results showed that the indexes of baguan group were significantly higher than that of the control group.The difference was statistically significant(P<0.05).Conclusion:Baguan plays a role in up-regulating the level of Mast Cell、NPY、5-HT in the local skin tissue at Huantiao(GB30).These neuroactive substances may convey signals to certain pathways that contribute to the effects of baguan.

关 键 词：拔罐 肥大细胞 神经肽Y 5-羟色胺 环跳 穴区效应 皮肤组织 实验研究 

分 类 号：R245[医药卫生—针灸推拿学]