三价铁对有机物存在下厌氧氨氧化脱氮的影响  

Effect of Fe^(3+) on nitrogen removal of Anammox in the presence of organic matter

作　　者：王海月 彭玲 毛念佳 耿金菊[1] 任洪强[1] 许柯 WANG Hai-yue;PENG Ling;MAO Nian-jia;GENG Jin-ju;REN Hong-qiang;XU Ke(State Key Laboratory of Pollution Control and Resource Reuse,School of the Environment,Nanjing University,Nanjing 210023,China)

机构地区：[1]南京大学环境学院,污染控制与资源再利用国家重点实验室,江苏南京210023

出　　处：《中国环境科学》2021年第4期1672-1680,共9页China Environmental Science

基　　金：水体污染控制与治理科技重大专项(2017ZX07202003)。

摘　　要：考察了三价铁(2.24~7.84mg/L)存在下厌氧氨氧化系统对有机物的耐受性能,并通过16SrRNA高通量测序技术和定量PCR探究其机理.结果表明,进水COD浓度为50和100mg/L时,4个反应器的氨氮和总氮去除率均较高(>90%),三价铁的强化作用不明显;进水COD浓度继续升高(150和200mg/L),厌氧氨氧化受到抑制,三价铁的强化作用逐渐增加;COD浓度为200mg/L时,添加三价铁(7.84mg/L)可将氨氮和总氮去除率由61.3%和79.8%(对照组)提升至71.2%和84.7%.16SrRNA高通量测序技术表明,有机物存在下,污泥微生物群落结构出现变化,主要表现为厌氧氨氧化菌丰度的降低及反硝化菌群的大量增殖,进水添加三价铁可提高浮霉菌(Planctomycetes)的丰度.定量PCR结果表明,三价铁能够提高厌氧氨氧化菌16S rRNA及功能基因hzsB的丰度.The tolerance of Anammox to organic matter in the presence of Fe^(3+)(2.24~7.84mg/L)was investigated by running four reactors(R1control,R22.24mg/L Fe^(3+),R34.48mg/L Fe^(3+),R47.84mg/L Fe^(3+)).The mechanism was clarified by using 16SrRNA high-throughput sequencing technology and qPCR.The results showed that high NH_(4)^(+)-N and TN removal rates(>90%)of all four reactors could be achieved at the influent COD concentration of 50 and 100mg/L,and the presence of Fe^(3+)did not show the obvious positive effect.As the influent COD concentration increased to 150 and 200mg/L,the performance of Anammox was inhibited and the positive effect of Fe^(3+)increased.When the influent COD concentration was 200mg/L,the addition of Fe^(3+)(7.84mg/L)increased the NH_(4)^(+)-N and TN removals from 61.3%and 79.8%(R1)to 71.2%and 84.7%,respectively.16SrRNA high-throughput sequencing results indicated the decrease of Anammox bacteria and the proliferation of denitrifying bacteria in the presence of organic matter.The presence of Fe^(3+) could increase the abundance of Planctomycetes.Fe^(3+) had promoting effects on the abundance of Anammox 16S rRNA and functional gene hzsB by qPCR data analysis.

关 键 词：三价铁 生物脱氮 厌氧氨氧化 厌氧颗粒污泥膨胀床 COD 

分 类 号：X703[环境科学与工程—环境工程]