BMSC外泌体调控STAT3/GAP43通路促进脊髓后索损伤大鼠感觉传导功能恢复  

BMSC Exosomes Promote Sensory Conduction Function Recovery via STAT3/GAP43 Axis Post Dorsal Column Injury

作　　者：张美玲 王新 李波 王志杰[5] 郭晓玲[6] 王凤艳[2] 修玉才[2] 王天仪[2,3] ZHANG Mei-ling;WANG Xin;LI Bo;WANG Zhi-jie;GUO Xiao-ling;WANG Feng-yan;XIU Yu-cai;WANG Tian-yi(Postgraduate School of Chengde Medical University,Chengde Hebei,067000,China;Department of Spine Surgery,981 Hospital of PLA Joint Support Force;Hebei Key Laboratory of Nerve Injury and Repair;Department of Orthopedics,Sun Yat-sen Memorial Hospital,Sun Yat-sen University;Department of Pediatric Internal Medicine,Affiliated Hospital of Chengde Medical College;Department of Neurology,981 Hospital of PLA Joint Support Force)

机构地区：[1]承德医学院研究生院,河北承德067000 [2]中国人民解放军联勤保障部队第九八一医院脊柱外科 [3]河北省神经损伤与修复重点实验室 [4]中山大学孙逸仙纪念医院骨科 [5]承德医学院附属医院儿内科 [6]中国人民解放军联勤保障部队第九八一医院神经内科

出　　处：《承德医学院学报》2021年第2期91-95,共5页Journal of Chengde Medical University

基　　金：河北省自然科学基金面上项目(H2020406027,H2017101030);中央军委后勤保障部卫生局全军医学科技青年培育计划成长项目(16QNP074);总后卫生部全军医学科技青年培育项目(13QNP017)。

摘　　要：目的探索骨髓间充质干细胞外泌体修复脊髓后索损伤大鼠感觉的功能。方法抽提大鼠骨髓间充质干细胞外泌体。将大鼠分为假手术组、PBS对照组、外泌体组,构建后索损伤模型。外泌体组经尾静脉注射外泌体,PBS对照组注射等量PBS。胶带移除实验和体感诱发电位评估感觉传导功能。免疫荧光染色观察脊髓后索形态。Western Blot检测STAT3蛋白和GAP43蛋白。结果免疫荧光染色显示所提取骨髓间充质干细胞可表达CD29和CD90。Western Blot显示抽提外泌体高表达HSP70与Alix。与PBS对照组相比,外泌体组NF-200染色阳性神经元累积光密度大,体感诱发电位波形与胶带移除实验潜伏期改善,STAT3与GAP43蛋白表达升高(P<0.05)。结论骨髓间充质干细胞外泌体可通过STAT3/GAP43通路修复后索损伤大鼠感觉功能。Objective To explore the feature of BMSC exosomeson sensory conduction function recovery post dorsal column injury.Methods BMSC exosomes were extracted from Wistar rats.36 rats were randomly divided into sham group,PBS control group,and exosomes group.The exosomes group was injected with exosomes via the tail vein,and the PBS control group was injected with the same amount of PBS.Tape removal test and somatosensory evoked potential were used to evaluate sensory conduction function.The spinal cord dorsal column was observed by immunofluorescence staining,and Western Blot was used to detect STAT3 and GAP43 protein expression.Results Immunofluorescence staining showed that the extracted BMSC could express CD29 and CD90.Western Blot showed that HSP70 and Alix were highly expressed in the extracted exosomes.Compared with the PBS control group,the exosomes group had a larger NF-200 staining area,and improved the waveform of somatosensory evoked potential and latency in tape removal test.The expression of STAT3 and GAP43 protein was significantly increased in exosomes group.Conclusion BMSC exosomes can promote the recovery of sensory conduction function of rats with spinal cord dorsal column lesion through STAT3/GAP43 pathway.

关 键 词：骨髓间充质干细胞 外泌体 脊髓损伤 STAT3 GAP43 

分 类 号：R651.2[医药卫生—外科学]