二氟沙星光敏损伤溶菌酶的十二烷基磺酸钠-聚丙烯酰胺凝胶电泳研究  

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis to study photooxidative damage induced by difloxacin to lysozyme

作　　者：李海霞[1] 刘艳成[2] 王文锋[2] LI Haixia;LIU Yancheng;WANG Wenfeng(Nanjing Tech University Pujiang Institute,Nanjing 211132,China;Shanghai Institute of Applied Physics,Chinese Academy of Sciences,Shanghai 201800,China)

机构地区：[1]南京工业大学浦江学院,南京211132 [2]中国科学院上海应用物理研究所,上海201800

出　　处：《辐射研究与辐射工艺学报》2021年第2期20-26,共7页Journal of Radiation Research and Radiation Processing

摘　　要：本文采用稳态光照的方法研究了二氟沙星药物对溶菌酶生物大分子的光敏损伤。研究发现,在315~375 nm波长的光照射下,二氟沙星药物能够光敏损伤溶菌酶类大分子物质。通过十二烷基磺酸钠-聚丙烯酰胺凝胶电泳分析光照产物发现,生物大分子的光敏损伤途径和损伤产物与二氟沙星药物的浓度、光照时间、光照气氛等条件密切相关。其他条件相同时,二氟沙星浓度越高,光照时间越长,光照对溶菌酶的损伤越严重;三种不同气氛(氮气、空气、氧气)条件下,氧气气氛对溶菌酶的损伤最严重。通过对不同气氛条件下损伤机理的研究发现,在氮气气氛条件下为Ⅰ型光敏损伤机制,在有氧气氛条件下是I型和Ⅱ型反应协同作用的结果,并以Ⅱ型反应为主。The photooxidative damage induced by difloxacin to lysozyme was studied using steady-state illumination. Difloxacin damaged lysozyme structure under 315~375 nm light irradiation. Sodium dodecyl sulfatepolyacrylamide gel electrophoresis(SDS-PAGE) revealed that the mechanisms and products of oxidative damage were related to the difloxacin concentration, time of irradiation, and ambient conditions. Longer irradiation time and higher difloxacin concentration lead to more serious damage to lysozyme. When incubated under nitrogen, air, or oxygen, the most serious damage to lysozyme was observed in an oxygen atmosphere. The type Ⅰ process was examined in a nitrogen-saturated solution, whereas both type Ⅰ and type Ⅱ were observed in an aerobic atmosphere,and type Ⅱ was the dominant process.

关 键 词：溶菌酶 二氟沙星 光敏损伤 十二烷基磺酸钠-聚丙烯酰胺凝胶电泳 

分 类 号：R818.74[医药卫生—放射医学] R852.7[医药卫生—临床医学]