电晕放电等离子体辐射对紫花苜蓿种子的影响  

Effects of corona discharge plasma radiation on alfalfa seeds

作　　者：徐文倩 栾欣昱 李一冰 陈浩 丁昌江[1,2] 宋智青 XUWenqian;LUAN Xinyu;LI Yibing;CHEN Hao;DING Changjiang;SONG Zhiqing(Center for Energy Conservation and Emission Reduction in Fermentation Industry of Inner Mongolia,Hohhot 010051,China;Department of Science,Inner Mongolia University of Technology,Hohhot 010051,China)

机构地区：[1]内蒙古自治区发酵产业节能减排工程技术研究中心,呼和浩特010051 [2]内蒙古工业大学理学院,呼和浩特010051

出　　处：《辐射研究与辐射工艺学报》2021年第2期45-54,共10页Journal of Radiation Research and Radiation Processing

基　　金：国家自然科学基金项目(51767020、52067017);内蒙古自治区科技计划项目(2020GG0280);内蒙古自治区自然科学基金项目(2019MS06025、2020MS01016)资助。

摘　　要：将紫花苜蓿种子分为两组,一组直接在电晕放电场中暴露处理,另一组用培养皿盖遮挡,以阻止离子风的刻蚀作用,可近似为非均匀电场单因素作用。结果表明:电晕放电等离子体辐射可以改变种皮化学结构,处理后种皮傅里叶变换红外光谱峰值在2856 cm^(-1)和1729 cm^(-1)处发生变化,表明种皮中的蜡、脂质、纤维素有一定降解。种子漂浮率和表观接触角测量结果表明,电晕放电等离子体辐射提高了紫花苜蓿种子的亲水性,无遮挡组较遮挡组亲水性变化更大,表明培养皿盖遮挡可以有效减少离子风的物理化学刻蚀,并且离子风比非均匀电场对种皮的影响更大。扫描电子显微镜结果表明,种皮刻蚀严重,种皮中的纤维素降解,表面开裂,从而提高了种子的吸水能力。电晕放电等离子体辐照过的种子苗高显著增加,表明离子风的物理化学刻蚀已转变为宏观的生物学效应。该研究为电晕放电等离子体辐射紫花苜蓿种子的表面改性和化学结构组成的分析提供了实验数据支持,并为揭示电晕放电场作用机理提供了思路。Alfalfa seeds were directly treated with corona discharge plasma radiation and compared with the control group,which was covered by a Petri dish,blocking the etching effect of ionic wind and thus simulating the effect of a non-uniform electric field.The results showed that corona discharge plasma radiation changed the chemical structure of the seed coat.The peak value of the Fourier transform infrared spectrum of the seed coat at 2856 cm‒1 and 1729 cm‒1 changed after treatment,indicating that the wax,lipids,and cellulose in the seed coat may have been degraded.Measurements of the seed floating rate and apparent contact angle in the treated seeds compared to the measurements in the control group indicated that corona discharge plasma radiation also improved the hydrophilicity of the alfalfa seeds.In the control group,the Petri dish cover effectively reduced the physicochemical etching by ionic wind;thus,it appears that ionic wind has a greater influence on the seed coat than does the non-uniform electric field.Scanning electron micrograph images showed that the etching of the seed coat in the treated seeds was severe;the cellulose of the seed coat was degraded,and the surface was cracked,which improved the water absorption capacity of the seed.The seedling height of the seeds treated with corona discharge plasma radiation increased significantly compared to that of the control group,indicating that the physicochemical etching of the ionic wind had resulted in a macroscopic biological effect.This study provides experimental data support for the analysis of surface modification and chemical structure composition of alfalfa seeds treated with corona discharge plasma radiation and also provides a basis for further study of the biological effect mechanism of the corona discharge field.

关 键 词：电晕放电等离子体 紫花苜蓿 亲水性 种皮微观结构 

分 类 号：Q691.5[生物学—生物物理学]