应用流式细胞术对柳属染色体倍性与基因组大小测定  

Chromosome Ploidy and Genome Size Determination of Salix Using Flow Cytometry

作　　者：任伟超[1] 徐姣[2] 樊锐锋[2] 刘美琦 于欣欣 王思嘉 马伟[1] Ren Weichao;Xu Jiao;Fan Ruifeng;Liu Meiqi;Yu Xinxin;Wang Sijia;Ma Wei(Heilongjiang University of Chinese Medicine,Harbin 150040,P.R.China)

机构地区：[1]黑龙江中医药大学药学院,哈尔滨150040 [2]黑龙江中医药大学,哈尔滨150040

出　　处：《东北林业大学学报》2021年第4期56-61,共6页Journal of Northeast Forestry University

基　　金：国家中医药管理局全国中药资源普查项目(GZY-KJS-2018-004);黑龙江中医药大学优秀创新人才领军人才项目(2018RCL09);黑龙江省拜泉县中药资源数据指导下中药材标准化种植精准扶贫示范研究(058021)。

摘　　要：以6种柳属植物的圆头柳(Salix capitata)、乌柳(Salix cheilophila)、五蕊柳(Salix pentandra)、三蕊柳(Salix triandra)、垂柳(Salix triandra)、杞柳(Salix integra)为研究对象,选取大豆(Glycine max)、黄瓜(Cucumis sativus)作为标准品;应用流式细胞术,采用单细胞液测量、混合细胞液测量2种测量方法对部分柳属植物染色体倍性测定和基因组大小进行评估,分析不同种柳属或同一种不同倍性的柳属与其基因组大小之间的关联性。结果表明:乌柳为二倍体,圆头柳、五蕊柳、三蕊柳、垂柳为四倍体。乌柳、圆头柳、五蕊柳、三蕊柳、垂柳、杞柳基因组大小,分别为300、640、580、610、580、300 Mb。研究结果可为6种柳属植物全基因组测序提供参考。Salix capitata, S. cheilophila, S. pentandra, S. triandra, S. triandra and S. integra were taken as the research objects with Glycine max and Cucumis sativus as references. With flow cytometry(FCM), genome size and chromosome ploidy of the six Salix samples were evaluated by two measurements(single cell fluid measurement and mixed cell fluid measurement). And the relevance of different species of Salix, or of the same species(with different ploidy) and their genome size were analyzed. S. cheilophila was diploid, and S. capitata, S. pentandra, S. triandra, and S. babylonica were tetraploid. The genome sizes of S. cheilophila, S. capitata, S. pentandra, S. triandra, S. babylonica and S. integra were 300, 640, 580, 610, 580 and 300 Mb, respectively. These results may provide basic reference for the whole genome sequencing of the six Salix plants.

关 键 词：柳属 染色体倍性 基因组大小 流式细胞技术 

分 类 号：S718.4[农业科学—林学] S722.3