微孔化羊ADM复合hUCMSC促进创面修复的机制研究  

Study on the mechanism of microporous sheep ADM combined with hUCMSC in promoting wound healing

作　　者：杨霄彦 郝春光 李俊亮 符雪 王凌峰 Yang Xiaoyan;Hao Chunguang;Li Junliang;Fu Xue;Wang Lingfeng(Department of Pathology, Third Affiliated Hospital of Inner Mongolia Medical University, Baotou 014010,China;Inner Mongolia Institute of Burn Research, Baotou 014010, China)

机构地区：[1]内蒙古医科大学第三附属医院病理科,包头014010 [2]内蒙古烧伤研究所,包头014010

出　　处：《中华损伤与修复杂志（电子版）》2021年第2期97-103,共7页Chinese Journal of Injury Repair and Wound Healing(Electronic Edition)

基　　金：内蒙古自治区自然科学基金(2014MS0823)。

摘　　要：目的探讨微孔化羊脱细胞真皮基质(ADM)复合人脐带间充质干细胞(hUCMSC)促进全层损伤皮肤创面愈合的治疗机制。方法将hUCMSC种植于微孔化羊ADM上共培养,形成复合生物敷料。选用72只裸鼠制作成皮肤全层损伤模型,按随机数字表法分为3组,每组24只,hUCMSC种植于微孔化羊ADM上治疗全层损伤创面组(hUCMSC+微孔化羊ADM组);羊ADM治疗全层损伤组(羊ADM组);碘伏纱布治疗全层损伤创面组(碘伏纱布组)。术后14、21、28 d检测各组裸鼠创面愈合率,运用qRT-PCR技术检测创面组织中Bax和Bcl-2的mRNA的表达,免疫组织化学染色检测创面愈合相关蛋白细胞胶原蛋白Ⅰ和血管内皮生长因子(VEGF)的表达。数据比较采用单因素方差分析和t检验。结果术后14 d,hUCMSC+微孔化羊ADM组的愈合率为(65.34±14.72)%,明显高于碘伏纱布组[(37.54±10.21)%],也高于羊ADM组[(49.08±11.16)%],差异均有统计学意义(t=19.52、14.72,P<0.05)。随着损伤创面的逐步愈合,术后28 d,hUCMSC+微孔化羊ADM组的愈合率为(98.63±15.41)%,明显高于羊ADM组[(81.74±16.27)%]和碘伏纱布组[(63.47±14.80)%],差异均有统计意义(t=-16.42、20.35,P<0.05)。Bax在hUCMSC+微孔化羊ADM组的创面组织中的表达显著降低,尤其在术后21 d的表达量为0.25±0.06,明显低于碘伏纱布组(0.53±0.16)和羊ADM组(0.41±0.12),差异均有统计学意义(t=3.52、-2.83,P<0.05)。Bcl-2在hUCMSC+微孔化羊ADM组的表达显著高于其他2组,尤其是在术后21 d的表达量为0.63±0.19,明显高于碘伏纱布组(0.34±0.09)和羊ADM组(0.46±0.13),差异均有统计学意义(t=5.31、-6.07,P<0.05)。免疫组织化学技术检测发现hUCMSC+微孔化羊ADM组胶原蛋白Ⅰ和VEGF的表达深棕色细胞虽然比羊ADM组和碘伏纱布组稍微有所增多,但是效果并不显著。结论hUCMSC+微孔化羊ADM复合敷料,通过携带hUCMSC更能促进皮肤全层损伤的愈合并减少凋亡细胞的产生。Objective To explore the therapeutic mechanism of microporous sheep acellular dermal matrix(ADM)combined with human umbilical cord mesenchymal stem cell(hUCMSC)to promote the healing of full-thickness wounds.Methods hUCMSC was co-cultured on microporous sheep ADM to form composite biological dressings.Seventy-two nude mice were selected to make a full-thickness skin injury model and randomly divide them into 3 groups,each group of 24.hUCMSC+microporous sheep ADM group,sheep ADM group and iodophor gauze group.The wound healing rate of each group was detected at 14,21 and 28 days after operation,qRT-PCR technique was used to detect the expression of Bax and Bcl-2 in wound tissue,and immunohistochemical technique was used to detect the expression of wound healing related proteins.Data were analyzed with one-way analysis of variance and t test.Results On the 14 days after operation,the healing rate of the hUCMSC+microporous sheep ADM group was(65.34±14.72)%,which was significantly higher than that of the Iodophor gauze group(37.54±10.21)%,and also higher than that of the sheep ADM group[(49.08±11.16)%],the differences were statistically significant(t=19.52,14.72;P<0.05).With the gradual healing of the wound,the healing rate of the hUCMSC+microporous sheep ADM group was(98.63±15.41)%at 28 days after operation,which was significantly higher than that of the sheep ADM group[(81.74±16.27)%]and the Lodophor gauze group[(63.47±14.80)%],the differences were statistically significant(t=-16.42,20.35;P<0.05).The expression of Bax in the wound tissue of the hUCMSC+microporous sheep ADM group was significantly reduced,especially on the 21 days after operation,the expression level was 0.25±0.06,which was significantly lower than the Lodophor gauze group(0.53±0.16)and sheep ADM group(0.41±0.12),the differences were statistically significant(t=3.52,-2.83;P<0.05).The expression of Bcl-2 in the hUCMSC+microporous sheep ADM group was significantly higher than that of the other two groups,especially at the 21 days after operat

关 键 词：小鼠 创伤和损伤 间质干细胞 微孔化 羊脱细胞真皮基质 伤口愈合 

分 类 号：R641[医药卫生—外科学]