鸭脂肪细胞中circ-FBLN2鉴定与功能预测  

Validation and function prediction of circ-FBLN2 in duck adipocytes

作　　者：胡晓丹 王来娣[1] 杨婷 白皓[1] 江勇 王志秀 王莎莎[1] 陈国宏[1] 常国斌[1] HU Xiaodan;WANG Laidi;YANG Ting;BAI Hao;JIANG Yong;WANG Zhixiu;WANG Shasha;CHEN Guohong;CHANG guobin(College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China)

机构地区：[1]扬州大学动物科学技术学院,江苏扬州225009

出　　处：《浙江农业学报》2021年第4期602-609,共8页Acta Agriculturae Zhejiangensis

基　　金：国家科技支撑计划(2015BAD03B03);国家自然科学基金(31802057);江苏省333人才工程。

摘　　要：Circular RNA(circRNA)是一类在转录过程中形成的首尾相接的环状非编码RNA,其在多种生物学过程以及疾病发生过程中发挥重要作用。基于前期鸭胚原代脂肪细胞分化过程中circRNA测序结果,本研究选择由FBLN2编码的circ-FBLN2进行验证分析。通过PCR扩增和焦磷酸测序技术获得接合序列,采用Rnase R消化实验进一步验证了circ-FBLN2的存在,采用核质分离技术得出circ-FBLN2主要存在于细胞质中,且其在脂肪细胞诱导分化后显著下调,在樱桃谷鸭各组织中的表达具有一定的差异,主要在脂肪和腿肌中表达。生物信息学分析结果显示,circ-FBLN2与预测到的靶标基因主要参与MAPK,Toll样受体,以及细胞因子与受体互作通路等信号通路,揭示其可能通过对靶基因的调控作用间接调控鸭脂肪细胞分化过程。Circular RNA(circRNA)is a kind of circular non-coding RNA characterized by head to tail connection during the transcription process,which plays an important role in many biological processes and diseases.Based on the results of circRNA sequencing during pre-adipocyte differentiation,circ-FBLN2 encoded by FBLN2 was selected for validation analysis.The conjugated sequence was obtained by PCR amplification and pyrosequencing.And circ-FBLN2 was further verified by RNase R digestion experiment.The results of nuclear and cytoplasmic separation experiment showed that circ-FBLN2 mainly existed in cytoplasm,and its expression level of duck adipocyte was significantly decreased after differentiation.There was a certain difference in the expression level of different tissues in Cherry Valley duck,it was mainly expressed in fat and leg muscle.Bioinformatics analysis showed that the predicted target genes and circ-FBLN2 were involved in MAPK,Toll-like receptor and cytokine-cytokine receptor interaction pathways and other signaling pathways.All these announced that circ-FBLN2 may indirectly regulate the differentiation of duck adipocytes by regulating the target genes.

关 键 词：circ-FBLN2 樱桃谷鸭 脂肪细胞 组织表达 

分 类 号：S834[农业科学—畜牧学]