芹菜茎基腐病病原菌鉴定、消长动态调查与防治药剂筛选  

Identification of causal agent and dynamics survey of celery stalk basal rot and screening of fungicides

作　　者：王国荣[1] 冯晓晓[2] 吴慧明[3] 曹婷婷 李倩 郑永利 WANG Guorong;FENG Xiaoxiao;WU Huiming;CAO Tingting;LI Qian;ZHENG Yongli(Xiaoshan Agricultural Technology Extension Center,Hangzhou 311203,China;Agricultural Experiment Station,Zhejiang University,Hangzhou 310058,China;College of Agriculture and Food Science,Zhejiang A&F University,Hangzhou 311300,China;Zhejiang Plant Protection Quarantine and Pesticide Management Station,Hangzhou 310020,China;College of Life Sciences,China Jiliang University,Hangzhou 310018,China;Zhejiang Agricultural Products Quality and Safety Center,Hangzhou 310003,China)

机构地区：[1]浙江省杭州市萧山区农业技术推广中心,浙江杭州311203 [2]浙江大学农业试验站,浙江杭州310058 [3]浙江农林大学农业与食品科学学院,浙江杭州311300 [4]浙江省植保检疫与农药管理总站,浙江杭州310020 [5]中国计量大学生命科学学院,浙江杭州310018 [6]浙江省农产品质量安全中心,浙江杭州310003

出　　处：《浙江农业学报》2021年第4期661-669,共9页Acta Agriculturae Zhejiangensis

基　　金：浙江省“三农六方”科技协作项目(CTZB-F180706LWZ-SNY1);浙江省重点研发计划(2020C02027)。

摘　　要：为探明芹菜茎基腐病科学防控技术,于2017—2018年在浙江省杭州市系统观测了芹菜茎基腐病田间病情自然消长动态;采取组织分离、形态和系统发育分析、菌丝悬浮液接种等方法对病原菌进行了分离鉴定和致病性测定;采用菌丝生长速率法测定了29种常用杀菌剂对芹菜茎基腐病病原菌的抑制率,并开展了相关田间药效试验。结果表明,芹菜茎基腐病在芹菜上普遍发生,6—9月是该病的发病高峰期;引起芹菜茎基腐病的病原菌为尖孢镰刀菌,甲基硫菌灵和戊唑醇及其复配制剂对芹菜茎基腐病菌的菌丝生长抑制效果最佳,75%肟菌·戊唑醇WDG或70%甲基硫菌灵WP对芹菜茎基腐病的田间防效较好。In order to characterize the causal agent and occurrence of celery stalk basal rot in Hangzhou,Zhejiang province and effective fungicides,the dynamic survey of celery stalk rot in field was investigated in 2017-2018,the pathogen was isolated and identified by tissue separation,morphological and phylogenetic analysis and its pathogenicity was determined by mycelial suspension inoculation,the inhibition of 29 common fungicides were determined by mycelial growth rate method,and field control experiment were conducted as well.The results showed that frequent occurrence of celery stalk basal rot was during June to September.Fusarium oxysporum was the pathogenic fungus that caused celery stalk basal rot.In vitro screening test showed that thiophanate-methyl and tebuconazoles fungicides had the best inhibitory effect on mycelial growth of pathogenic F.oxysporum,and field application of 75%trifloxystrobin·tebuconazole WDG and 70%thiophanate-methyl WP had better control effect on celery stalk basal rot.

关 键 词：芹菜茎基腐病 尖孢镰刀菌 杀菌剂 

分 类 号：S436.36[农业科学—农业昆虫与害虫防治]