白藜芦醇联合顺铂对神经胶质瘤细胞的作用  

Effects of resveratrol combined with cisplatin on glioma cell

作　　者：闫苗苗[1] 潘效红[1] 范艳玲 刘文英 魏光成[1] 耿枫 Yan Miaomiao;Pan Xiaohong;Fan Yanling;Liu Wenying;Wei Guangcheng;Geng Feng(Pharmaceutical College,Binzhou Medical College,Yantai 264003,China)

机构地区：[1]滨州医学院药学院,烟台264003

出　　处：《解剖学杂志》2021年第2期136-139,146,共5页Chinese Journal of Anatomy

基　　金：国家自然科学基金(21201020);山东省自然科学基金(ZR2017LC006);山东省高等学校科技计划(J15LC01);烟台市科技计划(2018XSCC050)。

摘　　要：目的:探讨白藜芦醇联合顺铂对神经胶质瘤细胞C6的作用。方法:不同浓度的白藜芦醇与顺铂单独联合作用于神经胶质瘤细胞C6,24、48 h和72 h后,CCK8法检测增殖,并选出合适浓度进行后续试验;不同浓度的药物作用于细胞24 h后,吖啶橙法观察细胞形态变化、流式细胞仪检测细胞凋亡、细胞周期和免疫印迹检测蛋白Bax、Bcl-2和p-Erk1/2变化。结果:白藜芦醇与顺铂能协同抑制神经胶质瘤细胞C6的增殖。联合用药后细胞周期抑制在S期。免疫印迹结果显示凋亡蛋白Bax的表达量增加,而凋亡抑制蛋白Bcl-2的表达量减少,细胞外调节蛋白激酶p-Erk1/2的表达量减少。结论:白藜芦醇与顺铂联合对神经胶质瘤细胞具有相互增敏作用,能够协同抑制细胞增殖,并通过Ras-Raf-MEK-Erk信号通路促进其凋亡。Objective:To explore the effects of combined administration of resveratrol and cisplatin on glioma cell C6.Methods:Glioma cell C6 was treated with different concentrations of resveratrol and cisplatin at 24,48 h and 72 h.Cell viability was detected by cell counting kit(CCK8).Appropriate concentrations were selected for subsequent experiments.Glioma cell C6 was treated with different concentrations of the drug at 24 h.The morphological changes of cells were observed by acridine orange staining.The apoptosis of cells and cell cycle were detected by flow cytometry.The changes of protein Bax,Bcl-2 and p-Erk1/2 were detected by Western blotting method.Results:Resveratrol and cisplatin could synergistically inhibit proliferation of glioma cell C6,and they could synergistically induce apoptosis.Western blotting revealed that the expression of Bax was increased,and the expression of Bcl-2 and extracellular regulatory protein p-Erk1/2 was decreased.Conclusion:The combination of resveratrol and cisplatin has mutual sensitization effect on glioma cells,which can synergistically inhibit cell proliferation and promote apoptosis by Ras-Raf-MEK-Erk signaling pathway.

关 键 词：白藜芦醇 顺铂 神经胶质瘤细胞C6 增殖 凋亡 

分 类 号：R285[医药卫生—中药学]