Transcriptome-wide effect of Salix SmSPR1 in etiolated seedling of Arabidopsis  

作　　者：Xiaoxia Liu Jianguo Zhang Li Xue Guodong Rao 

机构地区：[1]State Key Laboratory of Tree Genetics and Breeding,Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,People’s Republic of China [2]Collaborative Innovation Center of Sustainable Forestry in Southern China,Nanjing Forestry University,Nanjing 210037,People’s Republic of China [3]Key Laboratory of Tree Breeding and Cultivation,State Forestry Administration,Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,People’s Republic of China

出　　处：《Journal of Forestry Research》2021年第3期975-985,共11页林业研究（英文版）

基　　金：supported by The Fundamental Research Funds for the Central Non-profit Research Institution of Chinese Academy of Forestry(CAFYBB2018QB001)。

摘　　要：Microtubules and their regulatory proteins are involved in the regulation of plant cell morphology.SPIRAL1(SPR1),a plant-specific microtubule-binding protein,is critical in regulating the anisotropic growth of plant cells.Our previous study showed that overexpressed S alix SmS PR1 genes in Arabidopsis thaliana caused right-handed spiral elongation in etiolated seedlings,but there were no morphological differences between wild-type and transgenic seedlings under varied light conditions.We then studied the transcriptional regulation patterns in transgenic plants engineered with the S mSPR1 gene.Transcriptomic results showed that a large number of differentially expressed genes were involved in plant light signal reception,chlorophyll synthesis and photosystem structure.Eleven gene families with 42 photosynthesis-related genes and 6 light-responsive genes were involved in regulation of cell morphology.Our results showed that these genes in the SmSPR1-ox line were particularly down-regulated under dark conditions.In addition,33 TFs showed differences between S mSPR1-ox and wild-type lines.Taken together,the transcriptome analysis provides new insight into investigating the molecular mechanisms of light-induced cell morphological changes mediated by the microtubule binding protein SPR1.

关 键 词：Arabidopsis thaliana Cell anisotropism SmSPR1 Transcription factor Transcriptome analysis 

分 类 号：Q943.2[生物学—植物学]